PMID- 11804949
OWN - NLM
STAT- MEDLINE
DCOM- 20020423
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 66
IP  - 2
DP  - 2002 Feb
TI  - Expression of monocyte chemoattractant protein-1 and distribution of immune cell 
      populations in the bovine corpus luteum throughout the estrous cycle.
PG  - 361-6
AB  - This study characterizes the expression of monocyte chemoattractant protein-1 
      (MCP-1) and the relative distribution of immune cell populations in the bovine 
      corpus luteum throughout the estrous cycle. Immunodetectable MCP-1 was evident in 
      corpora lutea of cows at Days 6, 12, and 18 postovulation (Day 0 = ovulation, n = 
      4 cows/stage). Day 6 corpora lutea contained minimal MCP-1 that was confined 
      primarily to blood vessels. In contrast, relatively intense staining for MCP-1 
      was observed in corpora lutea from Days 12 and 18 postovulation. MCP-1 was again 
      most evident in the cells of the vasculature, but it was also observed 
      surrounding individual luteal cells, particularly by Day 18. An increase in 
      immunohistochemical expression of MCP-1 on Days 12 and 18 postovulation 
      corresponded with increases in MCP-1 mRNA and protein in corpora lutea as 
      determined by Northern blot analysis and ELISA. Monocytes and macrophages were 
      the most abundant immune cells detected in the bovine corpus luteum, followed by 
      CD8+ and CD4+ T lymphocytes. In all instances, Day 6 corpora lutea contained 
      fewer immune cells than corpora lutea from Days 12 and 18. In conclusion, 
      increased expression of MCP-1 was accompanied by the accumulation of immune cells 
      in the corpora lutea of cows during the latter half of the estrous cycle (Days 
      12-18 postovulation). These results support the hypothesis that MCP-1 promotes 
      immune cell recruitment into the corpus luteum to facilitate luteal regression. 
      These results also raise a provocative issue, however, concerning the recruitment 
      of immune cells several days in advance of the onset of luteal regression.
FAU - Townson, David H
AU  - Townson DH
AD  - Department of Animal and Nutritional Sciences, University of New Hampshire, 
      Durham, New Hampshire 03824-3590, USA.
FAU - O'Connor, Cindy L
AU  - O'Connor CL
FAU - Pru, James K
AU  - Pru JK
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (CD8 Antigens)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - CD8 Antigens/immunology
MH  - Cattle
MH  - Cell Count
MH  - Chemokine CCL2/*biosynthesis
MH  - Corpus Luteum/cytology/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Estrous Cycle/*immunology
MH  - Female
MH  - Immunity, Cellular/*physiology
MH  - Immunohistochemistry
MH  - Macrophages/immunology
MH  - Monocytes/immunology
MH  - Ovulation/physiology
MH  - Progesterone/blood
MH  - RNA, Messenger/biosynthesis
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - T-Lymphocytes, Helper-Inducer/immunology
EDAT- 2002/01/24 10:00
MHDA- 2002/04/24 10:01
CRDT- 2002/01/24 10:00
PHST- 2002/01/24 10:00 [pubmed]
PHST- 2002/04/24 10:01 [medline]
PHST- 2002/01/24 10:00 [entrez]
AID - 10.1095/biolreprod66.2.361 [doi]
PST - ppublish
SO  - Biol Reprod. 2002 Feb;66(2):361-6. doi: 10.1095/biolreprod66.2.361.