PMID- 11807881
OWN - NLM
STAT- MEDLINE
DCOM- 20020213
LR  - 20191210
IS  - 0148-7299 (Print)
IS  - 0148-7299 (Linking)
VI  - 107
IP  - 2
DP  - 2002 Jan 15
TI  - PRINS tandem labeling of satellite DNA in the study of chromosome damage.
PG  - 99-104
AB  - Tandem labeling of satellite DNA was proposed a few years ago (1) for evaluating 
      preferential chromosome breaks in the pericentromeric regions of mammalian 
      chromosomes, and (2) for distinguishing chromosome breaks from chromosome 
      segregation errors in interphase cells. In the presence of primers and modified 
      nucleotides, primed in situ labeling (PRINS) tags repetitive DNA sequences, and 
      serves as a useful alternative to fluorescence in situ hybridization (FISH). We 
      developed a two-color method for PRINS tandem labeling of centromeric and 
      pericentromeric sequences. The method, which appears to be more sensitive than 
      FISH, was used to assay micronuclei in mouse splenocytes and early spermatids, 
      and it provided insight into mechanisms of induction of chromosome damage in 
      these cells. We compared the sensitivity of this method and of a different 
      two-color approach, based on simultaneous labeling of centromeric and telomeric 
      sequences.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Russo, Antonella
AU  - Russo A
AD  - Department of Structural and Functional Biology, University of Insubria, Varese, 
      Italy. russo@bio.unipd.it
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - United States
TA  - Am J Med Genet
JT  - American journal of medical genetics
JID - 7708900
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - Animals
MH  - Centromere
MH  - *Chromosome Aberrations
MH  - DNA, Satellite/*chemistry
MH  - Mice
MH  - Primed In Situ Labeling/*methods
MH  - Sensitivity and Specificity
MH  - Telomere
EDAT- 2002/01/25 10:00
MHDA- 2002/02/14 10:01
CRDT- 2002/01/25 10:00
PHST- 2002/01/25 10:00 [pubmed]
PHST- 2002/02/14 10:01 [medline]
PHST- 2002/01/25 10:00 [entrez]
AID - 10.1002/ajmg.10102 [pii]
AID - 10.1002/ajmg.10102 [doi]
PST - ppublish
SO  - Am J Med Genet. 2002 Jan 15;107(2):99-104. doi: 10.1002/ajmg.10102.