PMID- 11807884
OWN - NLM
STAT- MEDLINE
DCOM- 20020213
LR  - 20190906
IS  - 0148-7299 (Print)
IS  - 0148-7299 (Linking)
VI  - 107
IP  - 2
DP  - 2002 Jan 15
TI  - Combined use of PRINS and FISH in the study of the dystrophin gene.
PG  - 115-8
AB  - The efficacy of fluorescence in situ hybridization (FISH) may be limited in 
      specific applications by low-resolution sensitivity. Primed in situ labeling 
      (PRINS) is based on specific hybridization of an unlabeled oligonucleotide with a 
      denatured template and synthesis of a single-strand DNA in situ. This method may 
      represent a powerful alternative to FISH for gene mapping because of its ability 
      to generate multiple independent signals within the same gene segment. We 
      investigated the specificity of signals produced by a modified PRINS protocol 
      combining a centromeric probe for the X-chromosome with specific primers for 3'- 
      and 5'-terminal regions of the dystrophin gene. In approximately 70% of nuclei 
      from male and female subjects, we detected one or two large signals (X-chromosome 
      centromere) and two or four smaller signals (the two regions of the dystrophin 
      gene). Specific hybridization of the oligonucleotides on Xp was demonstrated by 
      localization of the smaller (dystrophin) and larger (X-centromere) signals on the 
      same chromosome. Simultaneous hybridization with a centromeric probe and 
      gene-specific oligonucleotides allowed localization of PRINS signals, and 
      assessment of the specificity of the primers used for hybridization. This 
      approach could facilitate identification of female carriers of small intragenic 
      deletions in the dystrophin gene.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Cinti, Caterina
AU  - Cinti C
AD  - Istituto di Citomorfologia Normale e Patologica, CNR, Bologna, Italy. 
      cinti@area.bo.cnr.it
FAU - Stuppia, Liborio
AU  - Stuppia L
FAU - Maraldi, Nadir Mario
AU  - Maraldi NM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Med Genet
JT  - American journal of medical genetics
JID - 7708900
RN  - 0 (DNA Primers)
RN  - 0 (Dystrophin)
SB  - IM
CIN - Am J Med Genet. 2002 Jan 15;107(2):95-6. PMID: 11807879
MH  - Base Sequence
MH  - DNA Primers
MH  - Dystrophin/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Primed In Situ Labeling/*methods
MH  - X Chromosome
EDAT- 2002/01/25 10:00
MHDA- 2002/02/14 10:01
CRDT- 2002/01/25 10:00
PHST- 2002/01/25 10:00 [pubmed]
PHST- 2002/02/14 10:01 [medline]
PHST- 2002/01/25 10:00 [entrez]
AID - 10.1002/ajmg.10104 [pii]
AID - 10.1002/ajmg.10104 [doi]
PST - ppublish
SO  - Am J Med Genet. 2002 Jan 15;107(2):115-8. doi: 10.1002/ajmg.10104.