PMID- 11807886
OWN - NLM
STAT- MEDLINE
DCOM- 20020213
LR  - 20191210
IS  - 0148-7299 (Print)
IS  - 0148-7299 (Linking)
VI  - 107
IP  - 2
DP  - 2002 Jan 15
TI  - Primed in situ labeling (PRINS) for evaluation of gene deletions in cancer.
PG  - 123-6
AB  - Rearrangements involving the 13q14 and 17p13 chromosomal regions are often 
      observed in leukemias and lymphomas. These rearrangements are not always 
      identifiable cytogenetically. In more than 50% of cases, deletions occur at the 
      submicroscopic level and the karyotypes appear normal. Molecular cytogenetic 
      techniques such as fluorescence in situ hybridization (FISH) have accordingly 
      contributed to the identification of a variety of subtle rearrangements such as 
      those involving submicroscopic deletions. However, FISH is expensive, time 
      consuming, technically burdensome, and requires cloned DNA probes. A newer 
      technique, primed in situ labeling (PRINS), has been tested as a possible 
      alternative to FISH. To assess the utility and efficiency of the PRINS method in 
      the detection of RB1 and p53 deletions, we evaluated 10 patients with 
      hematological disorders and known rearrangements, i.e., deletions involving 13q14 
      and 17p13 regions. The data in these cases were validated against data obtained 
      with standard FISH probes. Our results indicate that PRINS could be used with 
      relative ease in cytogenetics laboratories and could serve as an alternative to 
      conventional FISH for defining deletions involving unique sequences.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Tharapel, Sugandhi A
AU  - Tharapel SA
AD  - Cytogenetics Reference Laboratory, Pathology and Laboratory Medicine Service, 
      V.A. Medical Center, Tennessee 38104, USA. sugandhi.tharapel@med.va.gov
FAU - Kadandale, Jayarama S
AU  - Kadandale JS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Am J Med Genet
JT  - American journal of medical genetics
JID - 7708900
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 17
MH  - DNA Primers
MH  - DNA Probes
MH  - *Gene Deletion
MH  - *Genes, Retinoblastoma
MH  - *Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Neoplasms/*genetics
MH  - Primed In Situ Labeling/*methods
EDAT- 2002/01/25 10:00
MHDA- 2002/02/14 10:01
CRDT- 2002/01/25 10:00
PHST- 2002/01/25 10:00 [pubmed]
PHST- 2002/02/14 10:01 [medline]
PHST- 2002/01/25 10:00 [entrez]
AID - 10.1002/ajmg.10105 [pii]
AID - 10.1002/ajmg.10105 [doi]
PST - ppublish
SO  - Am J Med Genet. 2002 Jan 15;107(2):123-6. doi: 10.1002/ajmg.10105.