PMID- 11810048
OWN - NLM
STAT- MEDLINE
DCOM- 20020307
LR  - 20180213
IS  - 0030-2414 (Print)
IS  - 0030-2414 (Linking)
VI  - 62
IP  - 1
DP  - 2002
TI  - Acridine orange excited by low-dose radiation has a strong cytocidal effect on 
      mouse osteosarcoma.
PG  - 85-93
AB  - The study was conducted to clarify the cytocidal effect of combination therapy 
      consisting of administration of acridine orange (AO), which is a photosensitizer, 
      and radiation therapy using in vitro and in vivo mouse osteosarcoma models. The 
      results revealed that AO combined with low-dose X-ray irradiation of about 1-5 Gy 
      had a strong cytocidal effect on the cultured mouse osteosarcoma cells regardless 
      of their chemosensitivity, and that this combination therapy inhibited growth of 
      the in vivo mouse osteosarcoma by induction of tumor necrosis. This effect was 
      inhibited by L-histidine, but not by mannitol. These findings suggested that AO 
      might be excited by X-rays and kill osteosarcoma cells through the release of 
      singlet oxygen, which is toxic to living cells. This mechanism is similar to that 
      of photodynamic therapy with AO.
CI  - Copyright 2002 S. Karger AG, Basel
FAU - Hashiguchi, Shin
AU  - Hashiguchi S
AD  - Department of Orthopedic Surgery, Kyoto Prefectural University of Medicine, 
      Kyoto, Japan.
FAU - Kusuzaki, Katsuyuki
AU  - Kusuzaki K
FAU - Murata, Hiroaki
AU  - Murata H
FAU - Takeshita, Hideyuki
AU  - Takeshita H
FAU - Hashiba, Mitsuoki
AU  - Hashiba M
FAU - Nishimura, Tsunehiko
AU  - Nishimura T
FAU - Ashihara, Tsukasa
AU  - Ashihara T
FAU - Hirasawa, Yasusuke
AU  - Hirasawa Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Oncology
JT  - Oncology
JID - 0135054
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/adverse effects/*radiation effects/*therapeutic use
MH  - Animals
MH  - Cell Size/drug effects/radiation effects
MH  - Cell Survival/drug effects/radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Lung Neoplasms/drug therapy/pathology/radiotherapy/secondary
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Neoplasm Transplantation
MH  - Osteosarcoma/*drug therapy/pathology/*radiotherapy
MH  - Photochemotherapy/*methods
MH  - Survival Rate
MH  - Time Factors
MH  - Tumor Cells, Cultured
MH  - X-Rays
EDAT- 2002/01/26 10:00
MHDA- 2002/03/08 10:01
CRDT- 2002/01/26 10:00
PHST- 2002/01/26 10:00 [pubmed]
PHST- 2002/03/08 10:01 [medline]
PHST- 2002/01/26 10:00 [entrez]
AID - ocl62085 [pii]
AID - 10.1159/000048251 [doi]
PST - ppublish
SO  - Oncology. 2002;62(1):85-93. doi: 10.1159/000048251.