PMID- 11810196
OWN - NLM
STAT- MEDLINE
DCOM- 20020307
LR  - 20220408
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 116
IP  - 6
DP  - 2001 Dec
TI  - Ultrastructural localisation of intramuscular expression of BDNF mRNA by 
      silver-gold intensified non-radioactive in situ hybridisation.
PG  - 545-51
AB  - A non-radioactive in situ hybridisation method is described for the detection of 
      low intramuscular levels of brain-derived neurotrophic factor (BDNF) mRNA at the 
      electron microscope level. Application of high-grade silver-gold intensification 
      of the diaminobenzidine end product of in situ hybridisation revealed that in 
      adult rat muscle the constitutive expression of muscular BDNF is confined to the 
      myofibres; satellite cells, Schwann cells, endothelial cells, fibroblasts or 
      axons do not appear to contribute to BDNF production in normal muscle. Although 
      muscular BDNF is a neurotrophic factor for innervating motoneurons and supposedly 
      released only at the motor endplates, the production of BDNF mRNA appears to 
      occur along the entire length of the myofibres and is not confined to nuclei in 
      the postsynaptic regions.
FAU - Liem, R S
AU  - Liem RS
AD  - Department of Cell Biology, Section Electron Microscopy, Graduate School for 
      Behaviour and Cognitive Neurosciences, University of Groningen, Oostersingel 
      69-II, 9713 EZ Groningen, The Netherlands. R.S.B.Liem@med.rug.nl
FAU - Brouwer, N
AU  - Brouwer N
FAU - Copray, J C
AU  - Copray JC
LA  - eng
PT  - Journal Article
DEP - 20011127
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/genetics/*metabolism
MH  - Gene Expression/*genetics
MH  - Immunohistochemistry
MH  - In Situ Hybridization/*methods
MH  - Microscopy, Electron
MH  - Muscle, Skeletal/*metabolism/ultrastructure
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2002/01/26 10:00
MHDA- 2002/03/08 10:01
CRDT- 2002/01/26 10:00
PHST- 2001/10/30 00:00 [accepted]
PHST- 2002/01/26 10:00 [pubmed]
PHST- 2002/03/08 10:01 [medline]
PHST- 2002/01/26 10:00 [entrez]
AID - 10.1007/s00418-001-0349-z [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2001 Dec;116(6):545-51. doi: 10.1007/s00418-001-0349-z. Epub 
      2001 Nov 27.