PMID- 11810473
OWN - NLM
STAT- MEDLINE
DCOM- 20020425
LR  - 20220408
IS  - 0918-4287 (Print)
IS  - 0918-4287 (Linking)
VI  - 33
IP  - 3
DP  - 2000
TI  - Collateral sprouting mechanism after end-to-side nerve repair in the rat.
PG  - 151-6
AB  - The collateral sprouting mechanisms of axons from an uninjured donor nerve after 
      end-to-side nerve repair was investigated in motor nerves of rats, with special 
      reference to the neurotrophins related to nerve regeneration. In addition, growth 
      cone formation at the tip of the regenerating nerve was examined. A transected 
      medial gastrocnemius nerve (MGN) was transferred to the side of an intact lateral 
      gastrocnemius nerve (LGN) using a Y-shaped silicone tube. At 3, 7, or 14 days 
      later, the MGN with the LGN was transected and was stained immunohistologically. 
      Expression of neurotrophin-3 (NT-3) and Trk C (receptor of NT-3) was most 
      significantly observed 3 days postoperatively around the site of coaptation. 
      Brain-derived neurotrophic factor (BDNF) and Trk B (receptor of BDNF) was weakly 
      detected at the coaptation site 3 days after-operation. Growth-associated protein 
      43 (GAP-43), which is a marker of growth cone formation, was observed at the site 
      of coaptation in the LGN 7 days postoperatively and in the MGN at the site of 
      coaptation at 14 days. We concluded that motor nerve regeneration due to 
      collateral sprouting of axons after end-to-side nerve repair is possible. We thus 
      demonstrated the involvement of at least one neurotrophin, NT-3, in the process 
      of collateral sprouting of motor nerves.
FAU - Yamauchi, T
AU  - Yamauchi T
AD  - Department of Orthopaedic Surgery, Nara Medical University, 840 Shijo-cho, 
      Kashihara, Nara 634-8522, Japan. toruyama@naramed-u.ac.jp
FAU - Maeda, M
AU  - Maeda M
FAU - Tamai, S
AU  - Tamai S
FAU - Tamai, M
AU  - Tamai M
FAU - Yajima, H
AU  - Yajima H
FAU - Takakura, Y
AU  - Takakura Y
FAU - Haga, S
AU  - Haga S
FAU - Yamamoto, H
AU  - Yamamoto H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Med Electron Microsc
JT  - Medical electron microscopy : official journal of the Clinical Electron 
      Microscopy Society of Japan
JID - 9430789
RN  - 0 (GAP-43 Protein)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Axons/*physiology
MH  - GAP-43 Protein/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Microscopy, Confocal
MH  - Muscle, Skeletal/*innervation/pathology
MH  - Nerve Growth Factors/*metabolism
MH  - *Nerve Regeneration
MH  - *Nerve Transfer
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 2002/01/26 10:00
MHDA- 2002/04/26 10:01
CRDT- 2002/01/26 10:00
PHST- 2000/07/12 00:00 [received]
PHST- 2000/11/10 00:00 [accepted]
PHST- 2002/01/26 10:00 [pubmed]
PHST- 2002/04/26 10:01 [medline]
PHST- 2002/01/26 10:00 [entrez]
AID - 10.1007/s007950000015 [doi]
PST - ppublish
SO  - Med Electron Microsc. 2000;33(3):151-6. doi: 10.1007/s007950000015.