PMID- 11811255
OWN - NLM
STAT- MEDLINE
DCOM- 20020626
LR  - 20170214
IS  - 1545-9683 (Print)
IS  - 1545-9683 (Linking)
VI  - 15
IP  - 2
DP  - 2001
TI  - Transplantation of genetically modified fibroblasts expressing BDNF in adult rats 
      with a subtotal hemisection improves specific motor and sensory functions.
PG  - 141-50
AB  - OBJECTIVE: We have previously reported that grafting fibroblasts genetically 
      modified to express brain-derived neurotrophic factor (BDNF) into a subtotal 
      cervical hemisection site that destroys the entire lateral funiculus will promote 
      regeneration of rubrospinal axons and growth of other axons, prevent atrophy and 
      death of axotomized red nucleus neurons, and improve forelimb use during 
      spontaneous vertical exploration. We have now extended these studies by using 
      additional sensorimotor tests to examine recovery. METHODS: The range of tests 
      used included those in which the intervention did not improve recovery, those in 
      which the intervention was associated with recovery, and those that showed little 
      deficit. The selected tasks tested both sensory and motor functions and both 
      forelimb and forelimb function. We used the open-field locomotor rating scale 
      (BBB), locomotion on a narrow beam, forelimb use during swimming, horizontal rope 
      walking, and a somatosensory asymmetry (patch-removal) test. After testing during 
      an 8-week recovery period, a second lesion was made just rostral to the initial 
      lesion/transplant site to test the role of the transplant in recovery. The rats 
      were then retested for a further 5 weeks after the repeated lesion. RESULTS: The 
      horizontal rope, swim, and patch-removal tests were reliably sensitive to the 
      subtotal hemisection injury. Fb/BDNF-transplanted animals recovered motor 
      functions on the horizontal rope-crossing test, and this recovery was abolished 
      by a second lesion just rostral to the first lesion/transplant. In the 
      patch-removal test, the latency to contact the affected limb was shorter in 
      Fb/BDNF-treated rats than in the control group, and this effect was completely 
      abolished by a second lesion. CONCLUSIONS: The rope-crossing and patch-removal 
      tests are particularly useful tasks for assessing the beneficial effects of 
      BDNF-expressing grafts in this injury model.
FAU - Kim, D
AU  - Kim D
AD  - NeuroDetective Inc., Quakertown, Pennsylvania, USA.
FAU - Schallert, T
AU  - Schallert T
FAU - Liu, Y
AU  - Liu Y
FAU - Browarak, T
AU  - Browarak T
FAU - Nayeri, N
AU  - Nayeri N
FAU - Tessler, A
AU  - Tessler A
FAU - Fischer
AU  - Fischer
FAU - Murray, M
AU  - Murray M
LA  - eng
GR  - NS24707/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neurorehabil Neural Repair
JT  - Neurorehabilitation and neural repair
JID - 100892086
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Denervation
MH  - Fibroblasts/*metabolism/*transplantation
MH  - Genetic Techniques
MH  - *Motor Activity
MH  - Postural Balance
MH  - Rats
MH  - Recovery of Function
MH  - *Sensation
MH  - Spinal Cord/pathology/*physiopathology/*surgery
MH  - Spinal Cord Diseases/pathology/*physiopathology/*surgery
MH  - Swimming
EDAT- 2002/01/29 10:00
MHDA- 2002/06/27 10:01
CRDT- 2002/01/29 10:00
PHST- 2002/01/29 10:00 [pubmed]
PHST- 2002/06/27 10:01 [medline]
PHST- 2002/01/29 10:00 [entrez]
AID - 10.1177/154596830101500207 [doi]
PST - ppublish
SO  - Neurorehabil Neural Repair. 2001;15(2):141-50. doi: 10.1177/154596830101500207.