PMID- 11811585
OWN - NLM
STAT- MEDLINE
DCOM- 20021113
LR  - 20170214
IS  - 1011-8942 (Print)
IS  - 1011-8942 (Linking)
VI  - 15
IP  - 2
DP  - 2001 Dec
TI  - Effect of the absence of heat shock protein 70.1 (hsp70.1) on retinal 
      photoreceptors in normal and rd mice.
PG  - 67-73
AB  - The purposes of this study are to elucidate the retinal changes of heat shock 
      protein 70.1 (hsp70.1) knockout mice and to compare them between in normal and in 
      retinal degeneration (rd) mice. Eyes of hsp70.1 wild type (+/+) and knockout 
      (-/-) mice in the C57BL/6 or FVB genetic backgrounds respectively, which were 
      reared in the normal environment, were examined by fundus photography, 
      electroretinography, light microscopy, terminal dUTP nick-end labeling (TUNEL) 
      stain, and immunohistochemistry. In C57BL/6 mice, fundus photography showed no 
      changes between hsp70.1+/+ and -/- mice at 1 and 6 months of age. 
      Electroretinographic examination showed a tendency of decreased amplitude of a- 
      and b-wave with aging in both genotype, but there were not different 
      statistically. The ratios of the thickness of inner nuclear and outer nuclear 
      layer to the retinal thickness were respectively decreased with aging in both 
      genotype, but there were not different statstically. TUNEL assay showed a few 
      positively labeled cells in the ganglion cell, inner nuclear and outer nuclear 
      layers and the immunohistochemistry showed no immunopositivity of hsp70 in the 
      inner segments of photoreceptor cell layer in both genotype. In rd mice, fundus 
      photography showed a narrowing of the retinal vessels at the age of 4 weeks, 
      however, there were no differences of retinal changes including pigment 
      epithelial layer in both genotype. Electroretinographic examination at the 
      postnatal 2, 3 and 4 weeks showed no differences between them. Loss of 
      photoreceptor cell and outer nuclear layers showed no differences in both 
      genotype. In conclusion, there were no differences of the retinal changes at 
      least under the normal environmental condition in hsp70.1+/+ and -/- mice. These 
      results show that hsp70.1-/- mice can be used to study the role of hsp70.1 to the 
      external stress to the retina.
FAU - Yu, Y S
AU  - Yu YS
AD  - Department of Ophthalmology, Seoul National University College of Medicine, Seoul 
      National University Hospital, Korea.
FAU - Heo, J H
AU  - Heo JH
FAU - Hwang, S W
AU  - Hwang SW
FAU - Kim, J H
AU  - Kim JH
FAU - Seo, J S
AU  - Seo JS
FAU - Khwarg, S I
AU  - Khwarg SI
FAU - Chung, H
AU  - Chung H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - Korean J Ophthalmol
JT  - Korean journal of ophthalmology : KJO
JID - 8804513
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Protozoan Proteins)
RN  - 0 (heat-shock protein 70.1)
SB  - IM
MH  - Animals
MH  - Electroretinography
MH  - Fundus Oculi
MH  - HSP70 Heat-Shock Proteins/*deficiency/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout/genetics
MH  - Photoreceptor Cells, Vertebrate/*metabolism
MH  - Protozoan Proteins/genetics
MH  - Reference Values
MH  - Retinal Degeneration/metabolism/*pathology/*physiopathology
EDAT- 2002/01/29 10:00
MHDA- 2002/11/26 04:00
CRDT- 2002/01/29 10:00
PHST- 2002/01/29 10:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/01/29 10:00 [entrez]
AID - 10.3341/kjo.2001.15.2.67 [doi]
PST - ppublish
SO  - Korean J Ophthalmol. 2001 Dec;15(2):67-73. doi: 10.3341/kjo.2001.15.2.67.