PMID- 11817537
OWN - NLM
STAT- MEDLINE
DCOM- 20020812
LR  - 20231213
IS  - 0962-8819 (Print)
IS  - 0962-8819 (Linking)
VI  - 10
IP  - 6
DP  - 2001 Dec
TI  - Co-suppression mediated virus resistance in transgenic tobacco plants harboring 
      the 3'-untranslated region of Andean potato mottle virus.
PG  - 489-99
AB  - Nicotiana tabacum plants were transformed with the 3'-untranslated region of the 
      Andean Potato Mottle Virus (APMoV) genome RNA-2. Three strategies were used: the 
      introduction of this region in sense and in antisense orientation and of a 
      fragment comprising the entire 3'-untranslated region from RNA-2 and part of the 
      CP22 coat protein sequence. The transgenic lines were inoculated with the virus 
      and different responses were observed, ranging from susceptibility to APMoV to 
      complete immunity to virus infection, in which neither the virion nor viral RNA 
      was detected in the inoculated leaf and leaves that emerged after inoculation. 
      The R1 progeny from different R0 lines also showed an array of virus resistance 
      phenotypes, which was not correlated with the zygotic state of the transgene. 
      Resistance was positively correlated with low levels of transgene mRNA 
      accumulation, indicating a co-suppression-mediated mechanism towards the 
      transgenic transcripts and APMoV viral RNA.
FAU - Vaslin, M F
AU  - Vaslin MF
AD  - LGMV, Departamento de Genetica, Universidade Federal do Rio de Janeiro (UFRJ), 
      Brazil.
FAU - Vidal, M S
AU  - Vidal MS
FAU - Alves, E D
AU  - Alves ED
FAU - Farinelli, L
AU  - Farinelli L
FAU - de Oliveira, D E
AU  - de Oliveira DE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - 0 (3' Untranslated Regions)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Genes, Plant
MH  - Homozygote
MH  - Immunity, Innate
MH  - Models, Genetic
MH  - Plant Viruses/*genetics
MH  - *Plants, Genetically Modified
MH  - RNA, Messenger/metabolism
MH  - RNA, Viral/genetics
MH  - Nicotiana/*genetics
MH  - Transgenes
EDAT- 2002/01/31 10:00
MHDA- 2002/08/13 10:01
CRDT- 2002/01/31 10:00
PHST- 2002/01/31 10:00 [pubmed]
PHST- 2002/08/13 10:01 [medline]
PHST- 2002/01/31 10:00 [entrez]
AID - 10.1023/a:1013015401546 [doi]
PST - ppublish
SO  - Transgenic Res. 2001 Dec;10(6):489-99. doi: 10.1023/a:1013015401546.