PMID- 11820293
OWN - NLM
STAT- MEDLINE
DCOM- 20020502
LR  - 20190922
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 766
IP  - 1
DP  - 2002 Jan 5
TI  - Therapeutic isoniazid monitoring using a simple high-performance liquid 
      chromatographic method with ultraviolet detection.
PG  - 181-7
AB  - Simultaneous measurement of isoniazid and its main acetylated metabolite 
      acetylisoniazid in human plasma is realized by high-performance liquid 
      chromatography. The technique used is evaluated by a factorial design of 
      validation that proved to be convenient for routine drug monitoring. Plasma 
      samples are deproteinized by trichloroacetic acid and then the analytes are 
      separated on a microBondapak C18 column (Waters). Nicotinamide is used as an 
      internal standard. The mobile phase is 0.05 M ammonium acetate buffer (pH 
      6)-acetonitrile (99:1, v/v). The detection is by ultraviolet absorbance at 275 
      nm. The validation, using the factorial design allows one to: (a) test the 
      systematic factors of bias (linearity and matrix effect); (b) estimate the 
      relative standard deviations (RSDs) related to extraction, measure and sessions 
      assay. The linearity is confirmed to be within a range of 0.5 to 8 microg/ml of 
      isoniazid and 1 to 16 microg/ml of acetylisoniazid. This method shows a good 
      repeatability for both extraction and measurement (RSD INH=3.54% and 3.32%; RSD 
      Ac.INH=0.00% and 5.97%), as well as a good intermediate precision (RSD INH=7.96%; 
      RSD Ac.INH=15.86%). The method is also selective in cases of polytherapy as many 
      drugs are associated (rifampicin, ethambutol, pyrazinamide, streptomycin). The 
      matrix effect (plasma vs. water) is negligible for INH (3%), but statistically 
      significant for Ac.INH (11%). The application of this validation design gave us 
      the possibility to set up an easy and suitable method for INH therapeutic 
      monitoring.
FAU - Moussa, L Ait
AU  - Moussa LA
AD  - Laboratoire de Toxicologie d'Urgence et de Suivi Therapeutique, Centre 
      Anti-Poisons du Maroc, Institut National d'Hygiene, Rabat.
FAU - Khassouani, C E
AU  - Khassouani CE
FAU - Soulaymani, R
AU  - Soulaymani R
FAU - Jana, M
AU  - Jana M
FAU - Cassanas, G
AU  - Cassanas G
FAU - Alric, R
AU  - Alric R
FAU - Hue, B
AU  - Hue B
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Antitubercular Agents)
RN  - V83O1VOZ8L (Isoniazid)
SB  - IM
MH  - Antitubercular Agents/*blood
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Monitoring/*methods
MH  - Humans
MH  - Isoniazid/*blood
MH  - Reproducibility of Results
MH  - Spectrophotometry, Ultraviolet/*methods
EDAT- 2002/02/01 10:00
MHDA- 2002/05/03 10:01
CRDT- 2002/02/01 10:00
PHST- 2002/02/01 10:00 [pubmed]
PHST- 2002/05/03 10:01 [medline]
PHST- 2002/02/01 10:00 [entrez]
AID - S0378-4347(01)00434-0 [pii]
AID - 10.1016/s0378-4347(01)00434-0 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jan 5;766(1):181-7. doi: 
      10.1016/s0378-4347(01)00434-0.