PMID- 11821149
OWN - NLM
STAT- MEDLINE
DCOM- 20020423
LR  - 20190901
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 40
IP  - 5
DP  - 2002 Apr
TI  - The non-competitive AMPA receptor antagonist (GYKI 52466) blocks 
      quisqualate-induced acetylcholine release from the rat hippocampus and striatum: 
      an in vivo microdialysis study.
PG  - 419-26
AB  - The effects of the non-N-methyl-D-aspartate (NMDA) agonist quisqualate (QUIS) and 
      selective AMPA/kainate receptor antagonist 1-(aminophenyl)-methyl-7, 
      8-methyilendioxy-5H-2,3-benzodiazepine (GYKI 52466) on the release of 
      acetylcholine (ACh) from the hippocampus and striatum of freely moving rats were 
      studied by transversal microdialysis. Acetylcholine level in the dialisate was 
      measured by the high performance liquid chromatography (HPLC) method with an 
      electrochemical detector. The QUIS (100 microM) perfused through the striatum 
      induced an increase of extracellular ACh level (250%) which lasted for over 1h 
      and gradually returned to basal values. Local perfusion of GYKI 52466 (10-100 
      microM) to the striatum did not change the basal release of ACh. GYKI 52466 (10 
      microM) administered together with QUIS (100 microM) in he striatum antagonized 
      the stimulant effect of QUIS on the ACh release. Local administration of the QUIS 
      (100 microM) through the microdialysis fiber implanted in the hippocampus, caused 
      a long lasting increase of extracellular hippocampal ACh level (360%) which was 
      reversed when the drug was withdrawn from the perfusion solution. The stimulant 
      effect of QUIS was antagonized by concomitant perfusion of GYKI (10 microM). No 
      effect was seen on the basal ACh release when GYKI (10-100 microM) was perfused 
      through the hippocampus. Local perfusion with tetrodotoxin (1 microM) decrease 
      the basal release of ACh and prevented the QUIS-induced increase of ACh both in 
      the hippocampus and striatum. Our in vivo neurochemical results indicate that 
      hippocampal and striatal cholinergic systems are regulated by non-NMDA (probably 
      AMPA) glutamatergic receptors located in the hippocampus and striatum.
FAU - Rakovska, Angelina
AU  - Rakovska A
AD  - Institute of Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev St., bl. 
      23, 1113 Sofia, Bulgaria. rakovska@bio25.bas.bg
FAU - Kiss, Janos P
AU  - Kiss JP
FAU - Raichev, Peter
AU  - Raichev P
FAU - Lazarova, Maria
AU  - Lazarova M
FAU - Kalfin, Reni
AU  - Kalfin R
FAU - Djambazova, Elena
AU  - Djambazova E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Anti-Anxiety Agents)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Receptors, AMPA)
RN  - 102771-26-6 (GYKI 52466)
RN  - 12794-10-4 (Benzodiazepines)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - 8OC22C1B99 (Quisqualic Acid)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/antagonists & inhibitors/*metabolism
MH  - Animals
MH  - Anti-Anxiety Agents/*pharmacology
MH  - *Benzodiazepines
MH  - Corpus Striatum/drug effects/*metabolism
MH  - Excitatory Amino Acid Agonists/*pharmacology
MH  - Excitatory Amino Acid Antagonists/*pharmacology
MH  - Hippocampus/drug effects/*metabolism
MH  - Male
MH  - Microdialysis
MH  - Quisqualic Acid/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, AMPA/*antagonists & inhibitors
MH  - Tetrodotoxin/pharmacology
EDAT- 2002/02/01 10:00
MHDA- 2002/04/24 10:01
CRDT- 2002/02/01 10:00
PHST- 2002/02/01 10:00 [pubmed]
PHST- 2002/04/24 10:01 [medline]
PHST- 2002/02/01 10:00 [entrez]
AID - S0197018601001036 [pii]
AID - 10.1016/s0197-0186(01)00103-6 [doi]
PST - ppublish
SO  - Neurochem Int. 2002 Apr;40(5):419-26. doi: 10.1016/s0197-0186(01)00103-6.