PMID- 11821988
OWN - NLM
STAT- MEDLINE
DCOM- 20020207
LR  - 20190901
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 23
IP  - 1
DP  - 2001 Jul
TI  - Interleukin-18 expression and modulation in human corneal epithelial cells.
PG  - 64-8
AB  - PURPOSE: The interferon-gamma-inducing factor Interleukin-18 (IL-18) is a 
      recently described cytokine that appears to have multiple important 
      pro-inflammatory effects including the induction of interferon-gamma (IFN-gamma) 
      by activated T-cells. The expression of IL-18 by human cornea has not been 
      previously reported. In the present study, we examine the possibility that human 
      corneal epithelial cells are capable of producing this leukocyte-activating 
      factor which may play an important role in IFN-gamma-dependent inflammation 
      responses in the cornea. METHODS: Northern blot analysis and ELISA were used to 
      investigate the in vitro expression of IL-18 mRNA and protein respectively in 
      primary (HCEC) and transformed human corneal epithelial cells (HCET). To 
      determine if IL-18 expression was modulated by pro-inflammatory mediators, cells 
      were treated with lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (PMA) 
      or synthetic double stranded RNA (poly dI : dC). IL-18 bioactivity was determined 
      in a leukocyte interferon-gamma induction assay and IL-18 was immunolocalized in 
      whole human cornea by immunohistochemistry using a specific anti-IL-18 antibody. 
      RESULTS: IL-18 mRNA and bioactive protein was constitutively expressed by human 
      corneal epithelial cells and upregulated by PMA, LPS and poly dI : dC. The 
      constitutive expression of IL-18 protein immunoreactivity was also demonstrated 
      in the epithelial cells of whole human cornea tissue. CONCLUSIONS: This is the 
      first study demonstrating that corneal epithelial cells are capable of producing 
      the IFN-gamma inducing factor IL-18. Increased bioactive corneal IL-18 production 
      can be induced by a number of pro-inflammatory agents and may play an important 
      role in initiating gamma-interferon-mediated inflammatory responses in the 
      cornea.
FAU - Burbach, G J
AU  - Burbach GJ
AD  - Department of Dermatology, Emory University, Atlanta, GA 30322, USA.
FAU - Naik, S M
AU  - Naik SM
FAU - Harten, J B
AU  - Harten JB
FAU - Liu, L
AU  - Liu L
FAU - Dithmar, S
AU  - Dithmar S
FAU - Grossniklaus, H
AU  - Grossniklaus H
FAU - Ward, S L
AU  - Ward SL
FAU - Armstrong, C A
AU  - Armstrong CA
FAU - Caughman, S W
AU  - Caughman SW
FAU - Ansel, J C
AU  - Ansel JC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Interleukin-18)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Polydeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 25853-45-6 (poly d(I-C))
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Blotting, Northern
MH  - Cell Line
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelium, Corneal/drug effects/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Interleukin-18/*biosynthesis/genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Polydeoxyribonucleotides/pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 2002/02/01 10:00
MHDA- 2002/02/08 10:01
CRDT- 2002/02/01 10:00
PHST- 2002/02/01 10:00 [pubmed]
PHST- 2002/02/08 10:01 [medline]
PHST- 2002/02/01 10:00 [entrez]
AID - 10.1076/ceyr.23.1.64.5425 [doi]
PST - ppublish
SO  - Curr Eye Res. 2001 Jul;23(1):64-8. doi: 10.1076/ceyr.23.1.64.5425.