PMID- 11822819
OWN - NLM
STAT- MEDLINE
DCOM- 20020724
LR  - 20191105
IS  - 1061-186X (Print)
IS  - 1026-7158 (Linking)
VI  - 9
IP  - 6
DP  - 2001
TI  - Bioactive polymers for biohybrid artificial pancreas.
PG  - 473-84
AB  - To address the solution for some of the obstacles, such as low insulin secretion, 
      limited lifespan and aggregation of transplanted islets, encountered in 
      developing a biohybrid artificial pancreas (BAP), polymeric materials including a 
      reversible polymeric extracellular matrix (ECM), crystallized glucagon-like 
      peptide-1, and oxygen carrying polymers, were prepared and their potential 
      utilities in designing a compact and rechargeable BAP were investigated. For a 
      synthetic, reversible ECM, high molecular weight N-isopropylacrylamide copolymer 
      with a small amount of acrylic acid (2 mole%) was synthesized by conventional 
      radical polymerization in benzene, and its aqueous solution above a critical 
      polymer concentration displayed a sol-gel transition temperature near 
      physiological temperature (33-35 degrees C) without noticeable hysteresis. The 
      physicochemical properties of the gel with islet compatibility proved that the 
      synthetic ECM is an appropriate matrix which can make a BAP rechargeable. 
      Glucagon-like peptide-1 (GLP-1, 7-37) is known to have a strong stimulatory 
      effect on insulin secretion, particularly at high glucose concentrations. When 
      zinc-crystallized GLP-1 was entrapped along with islets in a hollow fiber 
      macrocapsule device, insulin secretion was enhanced at a high glucose 
      concentration (300 mg/dl) with a >85% increase in insulin secretion after an 
      induction period. The cross-linked hemoglobin with difunctional PEO (Hb-C) was 
      prepared to increase the high molecular weight of Hb. This prevents diffusional 
      loss when enclosed in an immunoprotecting membrane. The Hb-C, entrapped in 
      microcapsules, enhanced insulin secretion and improved the viability of 
      microencapsulated islets by promoting oxygen supply to islets. The introduction 
      of the synthetic ECM, crystallized GLP-1, and Hb-C into a BAP may provide a basis 
      for designing a compact and rechargeable BAP (macrocapsule).
FAU - Chae, S Y
AU  - Chae SY
AD  - Department of Pharmaceutics and Pharmaceutical Chemistry/CCCD, University of 
      Utah, Salt Lake City 84112, USA.
FAU - Kim, S W
AU  - Kim SW
FAU - Bae, Y H
AU  - Bae YH
LA  - eng
GR  - DK56884/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Drug Target
JT  - Journal of drug targeting
JID - 9312476
RN  - 0 (Hemoglobins)
RN  - 0 (Insulin)
RN  - 0 (Peptide Fragments)
RN  - 0 (Polymers)
RN  - 0 (Protein Precursors)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
RN  - 9007-92-5 (Glucagon)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - *Bioartificial Organs
MH  - Cell Culture Techniques/methods
MH  - Crystallization
MH  - Extracellular Matrix/chemistry/*metabolism
MH  - Glucagon/administration & dosage/chemistry/*metabolism
MH  - Glucagon-Like Peptide 1
MH  - Hemoglobins/chemistry
MH  - Insulin/metabolism
MH  - Insulin Secretion
MH  - Islets of Langerhans/metabolism/physiology
MH  - Oxygen/metabolism
MH  - Pancreas/metabolism/*physiology
MH  - Peptide Fragments/administration & dosage/chemistry/*metabolism
MH  - Polyethylene Glycols/chemistry
MH  - Polymers/*administration & dosage/chemistry/*metabolism
MH  - Protein Precursors/administration & dosage/chemistry/*metabolism
MH  - Rats
MH  - Tissue Engineering/*methods
EDAT- 2002/02/02 10:00
MHDA- 2002/07/26 10:01
CRDT- 2002/02/02 10:00
PHST- 2002/02/02 10:00 [pubmed]
PHST- 2002/07/26 10:01 [medline]
PHST- 2002/02/02 10:00 [entrez]
AID - 10.3109/10611860108998781 [doi]
PST - ppublish
SO  - J Drug Target. 2001;9(6):473-84. doi: 10.3109/10611860108998781.