PMID- 11823515
OWN - NLM
STAT- MEDLINE
DCOM- 20020304
LR  - 20201212
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 168
IP  - 4
DP  - 2002 Feb 15
TI  - Innate direct anticancer effector function of human immature dendritic cells. I. 
      Involvement of an apoptosis-inducing pathway.
PG  - 1823-30
AB  - Dendritic cells (DCs) mediate cross-priming of tumor-specific T cells by 
      acquiring tumor Ags from dead cancer cells. The process of cross-priming would be 
      most economical and efficient if DCs also induce death of cancer cells. In this 
      study, we demonstrate that normal human in vitro generated immature DCs 
      consistently and efficiently induce apoptosis in cancer cell lines, freshly 
      isolated noncultured cancer cells, and normal proliferating endothelial cells, 
      but not in most normal cells. In addition, in vivo generated noncultured 
      peripheral blood immature DCs mediate similar tumoricidal activity as their in 
      vitro counterpart, indicating that this DC activity might be biologically 
      relevant. In contrast to immature DCs, freshly isolated monocytes (myeloid DC 
      precursors) and in vitro generated mature DCs are not cytotoxic or are less 
      cytotoxic, respectively, suggesting that DC-mediated killing of cancer cells is 
      developmentally regulated. Comparable cytotoxic activity is mediated by untreated 
      DCs, paraformaldehyde-fixed DCs, and soluble products of DCs, and is destructible 
      by proteases, indicating that both cell membrane-bound and secreted proteins 
      mediate this DC function. Overall, our data demonstrate that human immature DCs 
      are capable of inducing apoptosis in cancer cells and thus to both directly 
      mediate anticancer activity and initiate processing of cellular tumor Ags.
FAU - Janjic, Bratislav M
AU  - Janjic BM
AD  - University of Pittsburgh Cancer Institute, Department of Pathology, University of 
      Pittsburgh School of Medicine, Pittsburgh, PA 15231, USA.
FAU - Lu, Ganwei
AU  - Lu G
FAU - Pimenov, Alexei
AU  - Pimenov A
FAU - Whiteside, Theresa L
AU  - Whiteside TL
FAU - Storkus, Walter J
AU  - Storkus WJ
FAU - Vujanovic, Nikola L
AU  - Vujanovic NL
LA  - eng
GR  - 1-PO DE13059-01/DE/NIDCR NIH HHS/United States
GR  - R01 CA 82016/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Membrane Proteins)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - *Apoptosis
MH  - Caspases/physiology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/pharmacology
MH  - Cytotoxicity Tests, Immunologic
MH  - Dendritic Cells/classification/*immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Kinetics
MH  - Membrane Proteins/physiology
MH  - Monocytes/immunology
MH  - Neoplasms/*immunology/pathology
MH  - Signal Transduction
MH  - Stem Cells/immunology
MH  - Tumor Cells, Cultured
EDAT- 2002/02/02 10:00
MHDA- 2002/03/05 10:01
CRDT- 2002/02/02 10:00
PHST- 2002/02/02 10:00 [pubmed]
PHST- 2002/03/05 10:01 [medline]
PHST- 2002/02/02 10:00 [entrez]
AID - 10.4049/jimmunol.168.4.1823 [doi]
PST - ppublish
SO  - J Immunol. 2002 Feb 15;168(4):1823-30. doi: 10.4049/jimmunol.168.4.1823.