PMID- 11824812
OWN - NLM
STAT- MEDLINE
DCOM- 20020723
LR  - 20190922
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 766
IP  - 2
DP  - 2002 Jan 25
TI  - Analysis of N-glycosylation of phospholipase A2 from venom of individual bees by 
      microbore high-performance liquid chromatography-electrospray mass spectrometry 
      using an ion trap mass spectrometer.
PG  - 243-50
AB  - The N-linked oligosaccharides were released from the phospholipase A2 (PLA) with 
      glycopeptidases and reductively aminated with the chromophore, p-aminobenzoic 
      acid ethyl ester (ABEE). The ABEE-labeled oligosaccharides were separated by 
      microbore high-performance liquid chromatography (micro-HPLC) using a 
      reversed-phase column and analyzed by electrospray mass spectrometry. 
      Differentiation between alpha-1,3 and alpha-1,6 core-fucosylated glycans was 
      achieved by comparison the glycans released by glycopeptidases 
      peptide-N-glycanase A (PNase A) and peptide-N-glycanase F (PNase F). All N-linked 
      oligosaccharides except 3B and 3C could be identified in this approach. The 
      analysis of PLA oligosaccharides from the venom of individual bees indicated that 
      glycosylation patterns between the younger and the older bees were similar.
FAU - Lai, Chien-Chen
AU  - Lai CC
AD  - Department of Chemistry, National Taiwan University, Taipei.
FAU - Her, Guor-Rong
AU  - Her GR
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Bee Venoms)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
SB  - IM
MH  - Animals
MH  - Bee Venoms/*enzymology
MH  - Bees
MH  - Carbohydrate Sequence
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Glycosylation
MH  - Molecular Sequence Data
MH  - Phospholipases A/chemistry/*metabolism
MH  - Phospholipases A2
MH  - Spectrometry, Mass, Electrospray Ionization/instrumentation/*methods
MH  - Spectrophotometry, Ultraviolet
EDAT- 2002/02/05 10:00
MHDA- 2002/07/24 10:01
CRDT- 2002/02/05 10:00
PHST- 2002/02/05 10:00 [pubmed]
PHST- 2002/07/24 10:01 [medline]
PHST- 2002/02/05 10:00 [entrez]
AID - S0378-4347(01)00479-0 [pii]
AID - 10.1016/s0378-4347(01)00479-0 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jan 25;766(2):243-50. doi: 
      10.1016/s0378-4347(01)00479-0.