PMID- 11826404
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20161019
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 35
IP  - 2
DP  - 2002 Feb
TI  - Derivation, characterization, and phenotypic variation of hepatic progenitor cell 
      lines isolated from adult rats.
PG  - 315-24
AB  - Liver progenitor cells (LPCs) cloned from adult rat livers following allyl 
      alcohol injury express hematopoietic stem cell and early hepatic lineage markers 
      when cultured on feeder layers; under these conditions, neither mature hepatocyte 
      nor bile duct, Ito, stellate, Kupffer cell, or macrophage markers are detected. 
      These phenotypes have remained stable without aneuploidy or morphological 
      transformation after more than 100 population doublings. When cultured without 
      feeder layers, the early lineage markers disappear, and mature hepatocyte markers 
      are expressed; mature hepatocytic differentiation and cell size are also 
      augmented by polypeptide and steroidal growth factors. In contrast to hepatocytic 
      potential, duct-like structures and biliary epithelial markers are expressed on 
      Matrigel. Because they were derived without carcinogens or mutagens, these 
      bipotential LPC lines provide novel tools for models of cellular plasticity and 
      hepatocarcinogenesis, as well as lines for use in cellular transplantation, gene 
      therapy, and bioreactor construction.
FAU - Yin, Li
AU  - Yin L
AD  - Division of Experimental Pathology, Albany Medical College, Albany, NY, USA.
FAU - Sun, Mingzeng
AU  - Sun M
FAU - Ilic, Zoran
AU  - Ilic Z
FAU - Leffert, Hyam L
AU  - Leffert HL
FAU - Sell, Stewart
AU  - Sell S
LA  - eng
GR  - P41 RR001315/RR/NCRR NIH HHS/United States
GR  - 5 P42 ES10337-02/ES/NIEHS NIH HHS/United States
GR  - CA71390/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
RN  - 0 (Biomarkers)
RN  - 0 (Propanols)
RN  - 3W678R12M0 (allyl alcohol)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cell Differentiation
MH  - Cell Line
MH  - Clone Cells/cytology
MH  - Cytological Techniques
MH  - Diploidy
MH  - Female
MH  - Genome
MH  - Hematopoietic Stem Cells/metabolism
MH  - Hepatocytes/*cytology/drug effects/*physiology
MH  - Phenotype
MH  - Propanols/pharmacology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Stem Cells/*cytology/drug effects/*physiology
EDAT- 2002/02/05 10:00
MHDA- 2002/03/01 10:01
CRDT- 2002/02/05 10:00
PHST- 2002/02/05 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2002/02/05 10:00 [entrez]
AID - S027091390276014X [pii]
AID - 10.1053/jhep.2002.31355 [doi]
PST - ppublish
SO  - Hepatology. 2002 Feb;35(2):315-24. doi: 10.1053/jhep.2002.31355.