PMID- 11827353
OWN - NLM
STAT- MEDLINE
DCOM- 20020716
LR  - 20190922
IS  - 0043-1354 (Print)
IS  - 0043-1354 (Linking)
VI  - 36
IP  - 2
DP  - 2002 Jan
TI  - Nitrifying and heterotrophic population dynamics in biofilm reactors: effects of 
      hydraulic retention time and the presence of organic carbon.
PG  - 469-81
AB  - Two biofilm reactors operated with hydraulic retention times of 0.8 and 5.0 h 
      were used to study the links between population dynamics and reactor operation 
      performance during a shift in process operation from pure nitrification to 
      combined nitrification and organic carbon removal. The ammonium and the organic 
      carbon loads were identical for both reactors. The composition and dynamics of 
      the microbial consortia were quantified by fluorescence in situ hybridization 
      (FISH) with rRNA-targeted oligonucleotide probes combined with confocal laser 
      scanning microscopy, and digital image analysis. In contrast to past research, 
      after addition of acetate as organic carbon nitrification performance decreased 
      more drastically in the reactor with longer hydraulic retention time. FISH 
      analysis showed that this effect was caused by the unexpected formation of a 
      heterotrophic microorganism layer on top of the nitrifying biofilm that limited 
      nitrifiers oxygen supply. Our results demonstrate that extension of the hydraulic 
      retention time might be insufficient to improve combined nitrification and 
      organic carbon removal in biofilm reactors.
FAU - Nogueira, Regina
AU  - Nogueira R
AD  - Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, Am Hochanger 4, 
      Freising, Germany.
FAU - Melo, Luis F
AU  - Melo LF
FAU - Purkhold, Ulrike
AU  - Purkhold U
FAU - Wuertz, Stefan
AU  - Wuertz S
FAU - Wagner, Michael
AU  - Wagner M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Acetates)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (RNA, Ribosomal)
RN  - 7440-44-0 (Carbon)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Acetates/metabolism
MH  - Bacteria
MH  - *Biofilms
MH  - *Bioreactors
MH  - Carbon/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Oligonucleotide Probes
MH  - Oxygen/analysis/metabolism
MH  - Population Dynamics
MH  - Quaternary Ammonium Compounds/analysis/metabolism
MH  - RNA, Ribosomal/genetics
MH  - Water Movements
EDAT- 2002/02/06 10:00
MHDA- 2002/07/18 10:01
CRDT- 2002/02/06 10:00
PHST- 2002/02/06 10:00 [pubmed]
PHST- 2002/07/18 10:01 [medline]
PHST- 2002/02/06 10:00 [entrez]
AID - S0043-1354(01)00229-9 [pii]
AID - 10.1016/s0043-1354(01)00229-9 [doi]
PST - ppublish
SO  - Water Res. 2002 Jan;36(2):469-81. doi: 10.1016/s0043-1354(01)00229-9.