PMID- 11829347
OWN - NLM
STAT- MEDLINE
DCOM- 20020226
LR  - 20190513
IS  - 0022-3069 (Print)
IS  - 0022-3069 (Linking)
VI  - 61
IP  - 1
DP  - 2002 Jan
TI  - Accumulation of beta-amyloid precursor protein in axons correlates with CNS 
      expression of SIV gp41.
PG  - 85-90
AB  - Axonal damage represented by accumulation of beta-amyloid precursor protein 
      (beta-APP) develops in numerous central nervous system (CNS) diseases including 
      human immunodeficiency virus (HIV) infection. To study the underlying mechanisms 
      of axonal damage associated with HIV CNS infection, the amount of axonal beta-APP 
      immunostaining in the corpus callosum of 24 simian immunodeficiency virus 
      (SIV)-infected macaques and 3 control macaques was measured by computerized image 
      analysis. The amounts of beta-APP accumulation were then compared with time 
      post-inoculation, extent and character of CNS inflammation, and viral load in the 
      CNS measured by the amount of immunohistochemical staining for the viral 
      transmembrane protein gp41. Significant increases over control values were 
      present in 10 of 24 SIV-infected animals. SIV encephalitis was present in 9 of 
      the 10 animals with elevated beta-APP Increases in beta-APP correlated most 
      strongly with levels of SIV gp41 in the brain (p = 0.005), but significant 
      associations with macrophage infiltration and microglial activation (p = 0.04) 
      and infiltration by cytotoxic lymphocytes (p = 0.05) also were identified. These 
      data demonstrate that beta-APP accumulation in the white matter of SIV-infected 
      macaques develops during SIV infection in close correlation with levels of viral 
      replication and may serve as a sensitive marker of neuronal/axonal damage 
      mediated by viral proteins.
FAU - Mankowski, J L
AU  - Mankowski JL
AD  - Division of Comparative Medicine, Department of Pathology, Johns Hopkins 
      University School of Medicine, Baltimore, Maryland 21205, USA.
FAU - Queen, S E
AU  - Queen SE
FAU - Tarwater, P M
AU  - Tarwater PM
FAU - Fox, K J
AU  - Fox KJ
FAU - Perry, V H
AU  - Perry VH
LA  - eng
GR  - RR000116/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Retroviridae Proteins)
RN  - 0 (SIV envelope protein gp41)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Amyloid beta-Protein Precursor/*metabolism
MH  - Animals
MH  - Axons/*metabolism/pathology
MH  - Brain/*metabolism/pathology/*virology
MH  - Disease Models, Animal
MH  - Encephalitis, Viral/*metabolism/pathology/virology
MH  - Humans
MH  - Immunohistochemistry
MH  - Macaca nemestrina
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Retroviridae Proteins/genetics/*metabolism
MH  - Simian Acquired Immunodeficiency Syndrome/*metabolism/pathology/virology
MH  - Simian Immunodeficiency Virus/genetics/*physiology
MH  - Viral Envelope Proteins/genetics/metabolism
MH  - Viral Load
MH  - Virus Replication
EDAT- 2002/02/07 10:00
MHDA- 2002/02/28 10:01
CRDT- 2002/02/07 10:00
PHST- 2002/02/07 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2002/02/07 10:00 [entrez]
AID - 10.1093/jnen/61.1.85 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 2002 Jan;61(1):85-90. doi: 10.1093/jnen/61.1.85.