PMID- 11829738
OWN - NLM
STAT- MEDLINE
DCOM- 20020322
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 362
IP  - Pt 1
DP  - 2002 Feb 15
TI  - Structural and functional characterization of the human and mouse fibulin-1 gene 
      promoters: role of Sp1 and Sp3.
PG  - 41-50
AB  - Fibulin-1 is a multifunctional extracellular protein involved in diverse 
      biological processes including cardiovascular development, haemostasis and 
      cancer. To investigate the transcriptional regulation of the gene encoding 
      fibulin-1 we cloned and analysed about 4.0 kb of the 5'-flanking regions of both 
      the human and mouse fibulin-1 genes. The human and mouse fibulin-1 promoters 
      share little sequence similarity except for a short region of approx. 150-170 bp 
      immediately upstream of the translation start site. The conserved region contains 
      a TATA-like sequence (ATAATT) and multiple consensus binding sites for Sp1 and 
      activator protein 2 (AP-2). That the short conserved region in each gene confers 
      basal promoter activity is demonstrated by transient transfections of promoter 
      deletion constructs for both the human and mouse genes into cells that express 
      fibulin-1 constitutively. Co-transfections of promoter constructs with expression 
      plasmids for Sp1, Sp3 and Sp4 into Drosophila SL2 cells indicate that Sp1 and Sp3 
      are essential for transcriptional activation and that these two factors act 
      synergistically. Electrophoretic mobility-shift assays show that Sp1 and Sp3, but 
      not AP-2, bind to the basal promoter of the human fibulin-1 gene. The results 
      demonstrate the functional importance of Sp1 and Sp3 in regulating the expression 
      of the fibulin-1 gene.
FAU - Castoldi, Mirco
AU  - Castoldi M
AD  - Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, 233 
      South 10th Street, Philadelphia, PA 19107, U.S.A.
FAU - Chu, Mon-Li
AU  - Chu ML
LA  - eng
SI  - GENBANK/AY040588
SI  - GENBANK/AY040589
GR  - GM 55625/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (SP3 protein, human)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Sp3 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (fibulin)
RN  - 148710-94-5 (Sp3 Transcription Factor)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcium-Binding Proteins/*genetics
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*physiology
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Sequence Homology, Nucleic Acid
MH  - Sp1 Transcription Factor/*physiology
MH  - Sp3 Transcription Factor
MH  - Transcription Factors/*physiology
MH  - Transcription, Genetic
PMC - PMC1222358
EDAT- 2002/02/07 10:00
MHDA- 2002/03/23 10:01
PMCR- 2002/08/15
CRDT- 2002/02/07 10:00
PHST- 2002/02/07 10:00 [pubmed]
PHST- 2002/03/23 10:01 [medline]
PHST- 2002/02/07 10:00 [entrez]
PHST- 2002/08/15 00:00 [pmc-release]
AID - 10.1042/0264-6021:3620041 [doi]
PST - ppublish
SO  - Biochem J. 2002 Feb 15;362(Pt 1):41-50. doi: 10.1042/0264-6021:3620041.