PMID- 11829900
OWN - NLM
STAT- MEDLINE
DCOM- 20060310
LR  - 20131121
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 37
IP  - 8
DP  - 1999 Aug
TI  - [The effect of 1, 25 (OH)(2)D(3)on growth and apoptosis in breast cancer cell 
      line MCF-7].
PG  - 497-9
AB  - OBJECTIVE: To study the effect of 1, 25-dihydroxyvitamin D(3) [1, 25 (OH)(2)D(3)] 
      on growth and apoptosis in breast cancer cell line MCF-7. METHODS: We compared 
      cell numbers by using MTT method, analyzed cell cycle and apoptosis percentage by 
      flow cytometric, observed cellular structure and ultrastructure, determined 
      quantitatively apoptosis cells by terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL), and detected expression of bcl-2 protein by 
      Western Blot. RESULTS: Incubation with 1, 25 (OH)(2)D(3) 10(-7) mol/L, MCF-7 
      cells exhibited significant growth inhibition. Flow cytometric analysis indicated 
      cell's G(0)/G(1) arrest along with increasing apoptotic peak and percentage. The 
      action of cytotoxic drug Adr was strengthened. Western Blot showed that 1, 25 
      (OH)(2)D(3) could down-regulate bcl-2 protein expression. CONCLUSIONS: 1, 25 
      (OH)(2)D(3) could be a new hormone for the treatment of breast cancer.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of General Surgery, First Affiliated Hospital, Chongqing Medical 
      University, Chongqing 400016.
FAU - Yao, Z
AU  - Yao Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
RN  - 0 (Antineoplastic Agents, Hormonal)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Antineoplastic Agents, Hormonal/pharmacology
MH  - Apoptosis/*drug effects
MH  - Breast Neoplasms/metabolism/*pathology
MH  - Calcitriol/*analogs & derivatives/*pharmacology
MH  - Cell Cycle/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
EDAT- 2002/02/07 10:00
MHDA- 2006/03/11 09:00
CRDT- 2002/02/07 10:00
PHST- 2002/02/07 10:00 [pubmed]
PHST- 2006/03/11 09:00 [medline]
PHST- 2002/02/07 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 1999 Aug;37(8):497-9.