PMID- 11830477
OWN - NLM
STAT- MEDLINE
DCOM- 20020425
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 99
IP  - 4
DP  - 2002 Feb 15
TI  - Dramatic increase in lymph node dendritic cell number during infection by the 
      mouse mammary tumor virus occurs by a CD62L-dependent blood-borne DC recruitment.
PG  - 1282-8
AB  - Despite the information dealing with the differential phenotype and function of 
      the main mouse dendritic cell (DC) subpopulations, namely, CD8alpha(-) and 
      CD8alpha(+) DCs, their origin and involvement in antiviral immune responses in 
      vivo are still largely unknown. To address these issues, this study used the 
      changes occurring in DC subpopulations during the experimental infection by the 
      Swiss (SW) strain of the mouse mammary tumor virus (MMTV). MMTV(SW) induced an 
      18-fold increase in lymph node DCs, which can be blocked by anti-CD62L treatment, 
      concomitant with the presence of high numbers of DCs in the outer cortex, in 
      close association with high endothelial venules. These data suggest that the DC 
      increase caused by MMTV(SW) infection results from the recruitment of blood-borne 
      DCs via high endothelial venules, by a CD62L-dependent mechanism. In addition, 
      skin sensitization assays indicate that MMTV(SW) infection inhibits epidermal 
      Langerhans cell migration to the draining lymph node. Moreover, data on the 
      kinetics of MMTV(SW)-induced expansion of the different DC subsets support the 
      hypothesis that CD8(-) and CD8(+) DCs represent different maturation stages of 
      the same DC population, rather than myeloid- and lymphoid-derived DCs, 
      respectively, as previously proposed. Finally, the fact that DCs were infected by 
      MMTV(SW) suggests their participation in the early phases of infection.
FAU - Martin, Pilar
AU  - Martin P
AD  - Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, 
      Spain.
FAU - Ruiz, Sara Ruiz
AU  - Ruiz SR
FAU - del Hoyo, Gloria Martinez
AU  - del Hoyo GM
FAU - Anjuere, Fabienne
AU  - Anjuere F
FAU - Vargas, Hector Hernandez
AU  - Vargas HH
FAU - Lopez-Bravo, Maria
AU  - Lopez-Bravo M
FAU - Ardavin, Carlos
AU  - Ardavin C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CD8 Antigens)
RN  - 126880-86-2 (L-Selectin)
SB  - IM
MH  - Animals
MH  - CD8 Antigens/analysis
MH  - Cell Count
MH  - Cell Differentiation
MH  - Chemotaxis/*drug effects/immunology
MH  - Dendritic Cells/immunology/pathology/*virology
MH  - Immunophenotyping
MH  - L-Selectin/*pharmacology/physiology
MH  - Lymph Nodes/*pathology/virology
MH  - Lymphatic System
MH  - *Mammary Tumor Virus, Mouse
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Retroviridae Infections/immunology/*pathology
MH  - Tumor Virus Infections/immunology/*pathology
EDAT- 2002/02/07 10:00
MHDA- 2002/04/26 10:01
CRDT- 2002/02/07 10:00
PHST- 2002/02/07 10:00 [pubmed]
PHST- 2002/04/26 10:01 [medline]
PHST- 2002/02/07 10:00 [entrez]
AID - S0006-4971(20)38223-9 [pii]
AID - 10.1182/blood.v99.4.1282 [doi]
PST - ppublish
SO  - Blood. 2002 Feb 15;99(4):1282-8. doi: 10.1182/blood.v99.4.1282.