PMID- 11832777
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20061115
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 167
IP  - 3
DP  - 2002 Mar
TI  - Urinary concentration and tissue messenger RNA expression of monocyte 
      chemoattractant protein-1 as an indicator of the degree of hydronephrotic atrophy 
      in partial ureteral obstruction.
PG  - 1497-502
AB  - PURPOSE: To find a potential prognostic marker of the induction of hydronephrotic 
      atrophy in congenital hydronephrosis we investigated whether the messenger (m)RNA 
      expression and urinary concentration of monocyte chemoattractant protein-1 
      (MCP-1) correlated with the degree of partial ureteral obstruction, and 
      subsequent hydronephrotic atrophy and interstitial fibrosis. MATERIALS AND 
      METHODS: We created left partial ureteral obstruction in 96 juvenile Wistar rats 
      and complete ureteral obstruction in 18, while 16 underwent sham operation. 
      Depending on excretion of contrast medium into the renal pelvis after 3 days we 
      defined 2 degrees of hydronephrosis. Renal mRNA expression of MCP-1, and renal 
      pelvic and bladder urinary concentrations of MCP-1 were measured after 1, 2 and 3 
      weeks, and compared with the degree of hydronephrotic atrophy. RESULTS: Grade 1 
      partial ureteral obstruction resulted in mild histological changes. Grade 2 
      partial and complete obstruction resulted in significant hydronephrotic atrophy. 
      MCP-1 mRNA expression in the kidney remained unchanged in grade 1 partial 
      obstruction but was moderately increased in grade 2 partial obstruction and 
      clearly over expressed in complete ureteral obstruction. The renal pelvic urinary 
      concentration of MCP-1 was not higher in rats with grade 1 partial obstruction 
      than in sham operated animals but it was significantly increased in those with 
      grade 2 partial and complete obstruction. CONCLUSIONS: mRNA expression and the 
      urinary concentration of MCP-1 correlate with the degree of obstruction and 
      subsequent renal damage in hydronephrosis. They may serve as prognostic markers 
      in children with congenital hydronephrosis.
FAU - Stephan, Miriam
AU  - Stephan M
AD  - Department of Urology, University of Hamburg, Eppendorf University Hospital, 
      Hamburg, Germany.
FAU - Conrad, Stefan
AU  - Conrad S
FAU - Eggert, Thilo
AU  - Eggert T
FAU - Heuer, Roman
AU  - Heuer R
FAU - Fernandez, Salvador
AU  - Fernandez S
FAU - Huland, Hartwig
AU  - Huland H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Chemokine CCL2/*metabolism/urine
MH  - Hydronephrosis/*metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Prognosis
MH  - RNA, Messenger/*metabolism/urine
MH  - Rats
MH  - Rats, Wistar
MH  - Ureteral Obstruction/*metabolism
EDAT- 2002/02/08 10:00
MHDA- 2002/03/01 10:01
CRDT- 2002/02/08 10:00
PHST- 2002/02/08 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2002/02/08 10:00 [entrez]
AID - S0022-5347(05)65352-9 [pii]
PST - ppublish
SO  - J Urol. 2002 Mar;167(3):1497-502.