PMID- 11835533
OWN - NLM
STAT- MEDLINE
DCOM- 20020402
LR  - 20191114
IS  - 0887-8013 (Print)
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Linking)
VI  - 16
IP  - 1
DP  - 2002
TI  - Simple genotype analysis of the Asp299Gly polymorphism of the Toll-like 
      receptor-4 gene that is associated with lipopolysaccharide hyporesponsiveness.
PG  - 56-8
AB  - A nonsynonymous single nucleotide polymorphism (Asp299Gly) in the Toll-like 
      receptor-4 (TLR-4) gene affects the responsiveness to lipopolysaccharide in 
      humans. To analyze this important polymorphism more efficiently, we developed a 
      simple polymerase chain reaction (PCR) restriction length fragment polymorphism 
      (RFLP) assay and examined the Asp299Gly allele frequency in a Japanese 
      population. The PCR primer was designed with 1- or 2-bp mismatches, creating the 
      recognition sequence for restriction enzyme BsaBI or BstXI, allowing RFLP 
      analysis of the digested products. Genotyping was carried out with this assay for 
      275 DNA specimens from 107 healthy volunteers and 168 patients with various 
      diseases, including ulcerative colitis (n = 86). The Asp299Gly allele of the 
      TLR-4 gene was not detected in any of the specimens, suggesting that it is very 
      rare in Japanese.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Okayama, Naoko
AU  - Okayama N
AD  - Division of Clinical Laboratory, Yamaguchi University Hospital, Yamaguchi 
      University School of Medicine, Yamaguchi, Japan.
FAU - Fujimura, Kozue
AU  - Fujimura K
FAU - Suehiro, Yutaka
AU  - Suehiro Y
FAU - Hamanaka, Yuichiro
AU  - Hamanaka Y
FAU - Fujiwara, Motoki
AU  - Fujiwara M
FAU - Matsubara, Tomoyo
AU  - Matsubara T
FAU - Maekawa, Tsuyoshi
AU  - Maekawa T
FAU - Hazama, Shoichi
AU  - Hazama S
FAU - Oka, Masaaki
AU  - Oka M
FAU - Nohara, Hiroaki
AU  - Nohara H
FAU - Kayano, Kozo
AU  - Kayano K
FAU - Okita, Kiwamu
AU  - Okita K
FAU - Hinoda, Yuji
AU  - Hinoda Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Drosophila Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Colitis, Ulcerative/blood/*genetics
MH  - *Drosophila Proteins
MH  - Genotype
MH  - Humans
MH  - Lipopolysaccharides/metabolism
MH  - Membrane Glycoproteins/blood/*genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - Receptors, Cell Surface/blood/*genetics
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
PMC - PMC6807990
EDAT- 2002/02/09 10:00
MHDA- 2002/04/03 10:01
PMCR- 2002/01/08
CRDT- 2002/02/09 10:00
PHST- 2002/02/09 10:00 [pubmed]
PHST- 2002/04/03 10:01 [medline]
PHST- 2002/02/09 10:00 [entrez]
PHST- 2002/01/08 00:00 [pmc-release]
AID - 10.1002/jcla.2075 [pii]
AID - JCLA2075 [pii]
AID - 10.1002/jcla.2075 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2002;16(1):56-8. doi: 10.1002/jcla.2075.