PMID- 11835770
OWN - NLM
STAT- MEDLINE
DCOM- 20050902
LR  - 20081121
IS  - 0412-4081 (Print)
IS  - 0412-4081 (Linking)
VI  - 35
IP  - 1
DP  - 1999 Jan
TI  - [The apoptosis and proliferation after photorefractive keratectomy].
PG  - 29-32, 3
AB  - OBJECTIVE: To search the correlation between the apoptosis and proliferation of 
      keratocytes and investigate the influence of phototherapeutic keratectomy (PTK) 
      and mechanical epithelial scrape (MES) on keratocyte apoptosis and proliferation. 
      METHODS: Rabbit corneas received photorefractive keratectomy (PRK, -9.9 diopters, 
      6 mm diameter). Animals were evaluated subsequently up to 6 months after surgery 
      by in vivo confocal microscopy. Corneas were prepared for H.E. staining, corneal 
      cell apoptosis was evaluated by terminal deoxyribonucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) to detect DNA fragmentation. 
      RESULTS: Loss of keratocytes and keratocyte death were found anteriorly in the 
      remaining stroma at 4h after PRK. Activated keratocytes observed by confocal 
      microscopy repopulated within 1 month. A significant elevation of apoptosis was 
      detected in keratocytes and epithelium at 4h, 3d, 1m after PRK. The level of 
      keratocyte apoptosis was parallel to the loss of keratocytes at the early stage 
      and was correlated with keratocyte proliferation in the later stage, PTK-PRK was 
      associated with lower levels of central corneal apoptosis and activated 
      keratocytes than MES-PRK. CONCLUSION: It is suggested that the loss of 
      keratocytes and keratocyte apoptosis be correlated with keratocyte activation and 
      proliferation, which may result in haze and regression after PRK, and PTK-PRK 
      induce lower level of early keratocyte apoptosis and late keratocyte activation 
      than MES-PRK.
FAU - Li, Y
AU  - Li Y
AD  - Department of Ophthalmology, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences, Peking Union Medical College, Beijing 100730.
FAU - Pang, G
AU  - Pang G
FAU - Zhan, S
AU  - Zhan S
FAU - Jin, Y
AU  - Jin Y
FAU - Sun, Y
AU  - Sun Y
FAU - Li, Y
AU  - Li Y
FAU - Li, W
AU  - Li W
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yan Ke Za Zhi
JT  - [Zhonghua yan ke za zhi] Chinese journal of ophthalmology
JID - 16210540R
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Proliferation
MH  - Corneal Stroma/*pathology/surgery
MH  - Female
MH  - In Situ Nick-End Labeling
MH  - Lasers, Excimer
MH  - Male
MH  - Microscopy, Confocal
MH  - *Photorefractive Keratectomy
MH  - Rabbits
EDAT- 2002/02/12 10:00
MHDA- 2005/09/03 09:00
CRDT- 2002/02/12 10:00
PHST- 2002/02/12 10:00 [pubmed]
PHST- 2005/09/03 09:00 [medline]
PHST- 2002/02/12 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yan Ke Za Zhi. 1999 Jan;35(1):29-32, 3.