PMID- 11836300
OWN - NLM
STAT- MEDLINE
DCOM- 20020301
LR  - 20171116
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 87
IP  - 2
DP  - 2002 Feb
TI  - Brain-derived neurotrophic factor: a novel human ovarian follicular protein.
PG  - 655-9
AB  - Neurotrophins are a family of soluble polypeptide growth factors widely 
      recognized for their roles in the mammalian nervous system. One such 
      neurotrophin, brain-derived neurotrophic factor (BDNF) was originally described 
      in the nervous system but has now been shown to be expressed in a variety of 
      nonneuronal tissues including endocrine tissues. We examined the human ovarian 
      follicle for its possible secretion of BDNF and further studied mouse oocytes to 
      determine BDNF's possible influence upon oocyte maturation. In a series of 
      experiments derived from human specimens from in vitro fertilization following 
      oocyte retrieval, BDNF was detected in human follicular fluid. To define the 
      source of BDNF, cumulus granulosa cells (the cells that immediately surround the 
      developing oocyte) were grown in cell culture for 1-2 d. BDNF protein increased 
      over 24 h in the culture medium. Moreover, the release of BDNF was enhanced upon 
      stimulation with cAMP or forskolin, an activator of cAMP. In contrast, mural 
      granulosa (cells lining the follicle), oocytes, and embryos did not release 
      appreciable quantities of BDNF. To examine possible targets of BDNF, mouse 
      studies were used to localize the BDNF receptor, Trk B, immunocytochemically. The 
      receptor was present on the surface of isolated oocytes. Moreover, BDNF promoted 
      mouse oocyte maturation in culture. These experiments demonstrate for the first 
      time the presence and secretion of BDNF from follicular cells in the human ovary 
      and suggest a possible role for BDNF in the regulation and modulation of oocyte 
      maturation.
FAU - Seifer, David B
AU  - Seifer DB
AD  - Department of Obstetrics, Gynecology and Reproductive Sciences, University of 
      Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New 
      Brunswick, New Jersey 08901, USA.
FAU - Feng, Bo
AU  - Feng B
FAU - Shelden, Robert M
AU  - Shelden RM
FAU - Chen, Shiling
AU  - Chen S
FAU - Dreyfus, Cheryl F
AU  - Dreyfus CF
LA  - eng
GR  - AG-15425/AG/NIA NIH HHS/United States
GR  - HD23315/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - Adult
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Cellular Senescence/drug effects
MH  - Culture Techniques
MH  - Embryo, Mammalian
MH  - Female
MH  - Follicular Fluid/metabolism
MH  - Granulosa Cells/metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oocytes/drug effects/metabolism/physiology
MH  - Ovarian Follicle/cytology/*metabolism
MH  - Receptor, trkB/metabolism
EDAT- 2002/02/12 10:00
MHDA- 2002/03/02 10:01
CRDT- 2002/02/12 10:00
PHST- 2002/02/12 10:00 [pubmed]
PHST- 2002/03/02 10:01 [medline]
PHST- 2002/02/12 10:00 [entrez]
AID - 10.1210/jcem.87.2.8213 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2002 Feb;87(2):655-9. doi: 10.1210/jcem.87.2.8213.