PMID- 11836691
OWN - NLM
STAT- MEDLINE
DCOM- 20020405
LR  - 20061115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 19
IP  - 1
DP  - 2002 Feb
TI  - [The relationship of p53 gene mutation to cell differentiation and metastasis of 
      laryngeal squamous cell carcinoma].
PG  - 61-3
AB  - OBJECTIVE: To inquire about the relationship of p53 gene mutation to the 
      histopathological findings and clinical manifestation in cases of laryngeal 
      squamous cell carcinoma(LSCC). METHODS: The fresh samples from 60 cases of LSCC 
      were examined. Polymerase chain reaction and silver staining-single strand 
      conformation polymorphism (PCR-SSCP) and DNA direct sequencing were used to 
      detect the mutation of p53 gene in exons 5-8. RESULTS: The mutation rates were 
      69.2% and 85.3% in patients at clinical stage I-II and stage III-IV respectively 
      (P>0.05). In the well-, moderately- and poorly-differentiated cell of LSCC, the 
      mutation rates were 52.9%, 83.3% and 94.7% respectively (P<0.05). The p53 gene 
      mutation rate of LSCC patients with neck lymph-node metastasis was 96.4%, whereas 
      that of patients without neck lymph-node metastasis was 62.5% (P<0.05). Twenty 
      samples showed positive results in SSCP; 19 samples showed deletion and mutation 
      in codons 125-292 by DNA direct sequencing. CONCLUSION: The mutation of p53 gene 
      in exons 5-8 was closely related to cell differentiation and the neck lymph-node 
      metastasis of LSCC, but it was not related to the clinical stages of the LSCC 
      cases.
FAU - Zhang, Xiaoqing
AU  - Zhang X
AD  - Department of Otorhinolaryngology, the Fourth Affiliated Hospital, Sichuan 
      University, Chengdu, Sichuan, 610041 P. R. China. iooi@263.net
FAU - Wang, Lihong
AU  - Wang L
FAU - Liu, Shixi
AU  - Liu S
FAU - Ouyang, Xuesong
AU  - Ouyang X
FAU - Liang, Chuanyu
AU  - Liang C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/secondary
MH  - Cell Differentiation/*genetics
MH  - Exons
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Lymphatic Metastasis/genetics
MH  - *Mutation
MH  - Neoplasm Staging
MH  - Polymerase Chain Reaction/methods
MH  - Polymorphism, Single-Stranded Conformational
MH  - Sequence Analysis, DNA/methods
MH  - Tumor Suppressor Protein p53/*genetics
EDAT- 2002/02/12 10:00
MHDA- 2002/04/06 10:01
CRDT- 2002/02/12 10:00
PHST- 2002/02/12 10:00 [pubmed]
PHST- 2002/04/06 10:01 [medline]
PHST- 2002/02/12 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Feb;19(1):61-3.