PMID- 11836694
OWN - NLM
STAT- MEDLINE
DCOM- 20020405
LR  - 20061115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 19
IP  - 1
DP  - 2002 Feb
TI  - [Fluorescence in situ hybridization study on the chromosome 17 aneuploidy of 
      human oocytes failing to fertilize in vitro].
PG  - 72-5
AB  - OBJECTIVE: Compare two methods of preparing human unfertilized oocytes interphase 
      nucleus and analyze the relationship between the different in vitro 
      fertilization(IVF) indications, ovarian stimulation protocols, women's age and 
      frequency of chromosome 17 aneuploidy. METHODS: Tarkowski's air-drying method(3:1 
      methanol:acetic acid) and Coonen's 0.1% Tween 20/0.01 mol/L HCl method were used 
      to fix human unfertilized oocytes interphase nucleus, and telomeric probe of 
      17qter was used by standard fluorescence in situ hybridization(FISH) procedures 
      to confirm chromosome 17qter aneuploidy. RESULTS: Of 36 human unfertilized 
      oocytes, 24 were haploid (66.7%), 7 were disomic (19.44%), 5 were trisomic 
      (13.89%). The overall frequency of aneuploidy was 33.3%. There were no 
      differences between the protocols characterized by different maternal age, IVF 
      indication, ovarian stimulation. CONCLUSION: Tarkowski's air dry method is as 
      good as the method of Coonen's, but the latter method can avoid the smell 
      pollution of the methanol and acetic acid, and it is easy to operate. The 
      chromosome 17 aneuploidy is one of the factors to cause in vitro fertilization 
      failure of human oocytes.
FAU - Zhang, Qunfang
AU  - Zhang Q
AD  - Human Reproductive Engineering Laboratory, Xiangya Medical College, Central-South 
      University, Changsha, Hunan, 410078 P. R. China. lgxdirector@sina.com
FAU - Lu, Guangxiu
AU  - Lu G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Cell Nucleus/genetics/ultrastructure
MH  - Chromosomes, Human, Pair 17/*genetics/ultrastructure
MH  - DNA Probes
MH  - Diploidy
MH  - Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro/methods
MH  - Haploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Karyotyping
MH  - Microscopy, Fluorescence/methods
MH  - Ovulation Induction/methods
MH  - Ovum/*physiology
MH  - Trisomy
EDAT- 2002/02/12 10:00
MHDA- 2002/04/06 10:01
CRDT- 2002/02/12 10:00
PHST- 2002/02/12 10:00 [pubmed]
PHST- 2002/04/06 10:01 [medline]
PHST- 2002/02/12 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Feb;19(1):72-5.