PMID- 11839674
OWN - NLM
STAT- MEDLINE
DCOM- 20020528
LR  - 20041117
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 8
IP  - 2
DP  - 2002 Feb
TI  - Detection of genetic prognostic markers in uveal melanoma biopsies using 
      fluorescence in situ hybridization.
PG  - 534-9
AB  - PURPOSE: In uveal melanoma, specific chromosomal abnormalities are known to 
      correlate with the risk of metastases; changes in chromosomes 3 and 8q correlate 
      strongly with a decreased survival of the patient, whereas chromosome 6 
      abnormalities are associated with a better prognosis. Usually, karyotyping and 
      fluorescence in situ hybridization (FISH) analysis are used to detect these 
      abnormalities in resected tumor tissues. However, the evaluation of these 
      chromosomal changes is compromised in patients treated with eye-retaining 
      treatment protocols because of the lack of tumor material. The purpose of this 
      study was to validate the use of FISH for the analysis of genetic prognostic 
      markers. EXPERIMENTAL DESIGN: We analyzed 40 uveal melanoma fine needle 
      aspiration biopsies (FNABs) and the corresponding main tumor with FISH. RESULTS: 
      All biopsies were found to contain tumor cells, and FISH analyses of the samples 
      were successful in all cases. Statistical analysis showed very good agreement 
      between the FISH results from the biopsies and those from the main tumor. In only 
      2 of 249 hybridizations did we find a small variation that could have led to a 
      misclassification. CONCLUSIONS: Our results indicate that the application of FISH 
      to FNABs is a reliable method for assaying genetic prognostic parameters such as 
      chromosome 3 loss and/or chromosome 8q gain. Implementation of this method in a 
      diagnostic setting means that we are able to identify patients at risk of 
      developing metastatic disease, not only in enucleated patients but also in cases 
      treated with conservative treatment modalities such as radiotherapy.
FAU - Naus, Nicole C
AU  - Naus NC
AD  - Department of Ophthalmology, Erasmus University Rotterdam, 3000 DR Rotterdam, the 
      Netherlands.
FAU - Verhoeven, Anette C A
AU  - Verhoeven AC
FAU - van Drunen, Ellen
AU  - van Drunen E
FAU - Slater, Rosalyn
AU  - Slater R
FAU - Mooy, Cornelia M
AU  - Mooy CM
FAU - Paridaens, Dion A
AU  - Paridaens DA
FAU - Luyten, Gregorius P M
AU  - Luyten GP
FAU - de Klein, Annelies
AU  - de Klein A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 3
MH  - Chromosomes, Human, Pair 6
MH  - Chromosomes, Human, Pair 8
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Male
MH  - Melanoma/*genetics/*pathology
MH  - Middle Aged
MH  - *Prognosis
MH  - Uveal Neoplasms/*genetics/*pathology
EDAT- 2002/02/13 10:00
MHDA- 2002/05/29 10:01
CRDT- 2002/02/13 10:00
PHST- 2002/02/13 10:00 [pubmed]
PHST- 2002/05/29 10:01 [medline]
PHST- 2002/02/13 10:00 [entrez]
PST - ppublish
SO  - Clin Cancer Res. 2002 Feb;8(2):534-9.