PMID- 11841161
OWN - NLM
STAT- MEDLINE
DCOM- 20020718
LR  - 20190922
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 111
IP  - 1-3
DP  - 2001
TI  - Characterization of sex chromosomes in rainbow trout and coho salmon using 
      fluorescence in situ hybridization (FISH).
PG  - 125-31
AB  - With the aim of characterizing the sex chromosomes of rainbow trout (Oncorhynchus 
      mykiss) and to identify the sex chromosomes of coho salmon (O. kisutch), we used 
      molecular markers OmyP9, 5S rDNA, and a growth hormone gene fragment (GH2), as 
      FISH probes. Metaphase chromosomes were obtained from lymphocyte cultures from 
      farm specimens of rainbow trout and coho salmon. Rainbow trout sex marker OmyP9 
      hybridizes on the sex chromosomes of rainbow trout, while in coho salmon, 
      fluorescent signals were localized in the medial region of the long arm of one 
      subtelocentric chromosome pair. This hybridization pattern together with the 
      hybridization of a GH2 intron probe on a chromosome pair having the same 
      morphology, suggests that a subtelocentric pair could be the sex chromosomes in 
      this species. We confirm that in rainbow trout, one of the two loci for 5S rDNA 
      genes is on the X chromosome. In males of this species that lack a heteromorphic 
      sex pair (XX males), the 5S rDNA probe hybridized to both subtelocentrics. This 
      finding is discussed in relation to the hypothesis of intraspecific polymorphism 
      of sex chromosomes in rainbow trout.
FAU - Iturra, P
AU  - Iturra P
AD  - Facultad de Medicina, Programa de Genetica Humana, ICBM, Universidad de Chile, 
      Casilla, Santiago. piturra@machi.med.uchile.cl
FAU - Lam, N
AU  - Lam N
FAU - de la Fuente, M
AU  - de la Fuente M
FAU - Vergara, N
AU  - Vergara N
FAU - Medrano, J F
AU  - Medrano JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - DNA Probes
MH  - DNA, Ribosomal/genetics
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Oncorhynchus kisutch/*genetics
MH  - Oncorhynchus mykiss/*genetics
MH  - *Sex Chromosomes
EDAT- 2002/02/14 10:00
MHDA- 2002/07/19 10:01
CRDT- 2002/02/14 10:00
PHST- 2002/02/14 10:00 [pubmed]
PHST- 2002/07/19 10:01 [medline]
PHST- 2002/02/14 10:00 [entrez]
AID - 10.1023/a:1013725717142 [doi]
PST - ppublish
SO  - Genetica. 2001;111(1-3):125-31. doi: 10.1023/a:1013725717142.