PMID- 11841184
OWN - NLM
STAT- MEDLINE
DCOM- 20020718
LR  - 20190922
IS  - 0016-6707 (Print)
IS  - 0016-6707 (Linking)
VI  - 111
IP  - 1-3
DP  - 2001
TI  - Extensive polymorphism and chromosomal characteristics of ribosomal DNA in a 
      loach fish, Cobitis vardarensis (Ostariophysi, Cobitidae) detected by different 
      banding techniques and fluorescence in situ hybridization (FISH).
PG  - 413-22
AB  - When surveying the karyotype diversity of European loaches of the genus Cobitis 
      to identify species involved in hybrid polyploid complexes, an extensive 
      polymorphism in number and location of NORs was discovered in C. vardarensis 
      using Ag-staining, C-banding, CMA3-fluorescence and fluorescence in situ 
      hybridization (FISH). This species had 2n = 50, the karyotype contained 13 pairs 
      of metacentric, 10 pairs of submetacentric and two pairs of subtelocentric 
      chromosomes. The NOR-bearing chromosomes included one medium-sized metacentric 
      pair with a large CMA3-positive heterochromatic pericentromeric block, one small 
      metacentric as well as one large submetacentric pairs. Ribosomal sites were 
      always located in telomeres of these chromosomes. Each of the pair of NOR-bearing 
      chromosomes occurred in three variants - (1) presence and/or (2) absence of NORs 
      on both homologues and (3) heterozygous combination where only one of the 
      homologues bears NORs. Altogether, 10 different NOR cytotypes from 27 
      theoretically possible ones were discovered among 20 indviduals examined. The 
      number of NORs ranged from two to five per specimen. The results regarding the 
      number and locations of NORs as revealed by banding techniques were confirmed 
      using FISH with rDNA probe. NOR sites were of CMA3-positive, suggesting that 
      ribosomal sites are associated with GC-rich DNA. Very similar structural 
      polymorphism with multiple NORs is expressed in the Danubian loach C. 
      elongatoides indicating a close relationship between both species.
FAU - Rabova, M
AU  - Rabova M
AD  - Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech 
      Academy of Sciences, Libechov, Czech Republic.
FAU - Rab, P
AU  - Rab P
FAU - Ozouf-Costaz, C
AU  - Ozouf-Costaz C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - *Chromosomes
MH  - Cypriniformes/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Nucleolus Organizer Region
MH  - *Polymorphism, Genetic
EDAT- 2002/02/14 10:00
MHDA- 2002/07/19 10:01
CRDT- 2002/02/14 10:00
PHST- 2002/02/14 10:00 [pubmed]
PHST- 2002/07/19 10:01 [medline]
PHST- 2002/02/14 10:00 [entrez]
AID - 10.1023/a:1013763903513 [doi]
PST - ppublish
SO  - Genetica. 2001;111(1-3):413-22. doi: 10.1023/a:1013763903513.