PMID- 11846444
OWN - NLM
STAT- MEDLINE
DCOM- 20020520
LR  - 20171116
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 26
IP  - 2
DP  - 2002
TI  - Caffeine and other xanthines as cytochemical blockers and removers of 
      heterocyclic DNA intercalators from chromatin.
PG  - 145-54
AB  - Caffeine (CAF) and other xanthines non-covalently bind with the cationic 
      fluorescent dye acridine orange (AO) and with other heterocyclic mutagens and 
      carcinogens that are known to intercalate into double-stranded DNA (dsDNA). 
      Fluorescence microscopy and spectrofluorometry studies were employed to test the 
      ability of caffeine and certain other methyl substituted xanthines, with 
      different binding affinities for AO, to inhibit and to reverse the intercalation 
      of AO and other heterocyclic agents from intercalation with the DNA of nuclear 
      chromatin of air-dried cells. Results indicated that xanthines with binding 
      affinity for AO greater than 150 m(-1) block the AO molecule in a concentration 
      dependent manner and comply with mass action kinetics. Thus CAF and other 
      xanthines can be used to either inhibit intercalation of AO into nuclear DNA or 
      to remove AO once intercalated into nuclear DNA. The interactions between other 
      planar heterocyclics, xanthines, and nuclear chromatin dsDNA were also found to 
      be non-covalent. Studies are needed to determine the ability of CAF and other 
      xanthines to block and/or remove polyaromatic hydrocarbon (PAH) intercalators 
      from the DNA of living cells.
CI  - Copyright 2002 Elsevier Science Ltd.
FAU - Lyles, Mark B
AU  - Lyles MB
AD  - Department of Cellular and Structural Biology, The University of Texas Health 
      Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
FAU - Cameron, Ivan L
AU  - Cameron IL
LA  - eng
GR  - DE00152/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Chromatin)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Intercalating Agents)
RN  - 0 (Mutagens)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 1AVZ07U9S7 (Xanthine)
RN  - 268B43MJ25 (Uric Acid)
RN  - 27Y3KJK423 (Aminophylline)
RN  - 3G6A5W338E (Caffeine)
RN  - 9007-49-2 (DNA)
RN  - EJ939L81MY (1,3,7,9-tetramethyluric acid)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacology
MH  - Aminophylline/pharmacology
MH  - Animals
MH  - Caffeine/*pharmacology
MH  - Cell Nucleus/metabolism
MH  - Central Nervous System Stimulants/*pharmacology
MH  - Chick Embryo
MH  - Chromatin/*metabolism
MH  - Chromosomes/metabolism
MH  - DNA/*drug effects/*metabolism
MH  - Fluorescent Dyes/pharmacology
MH  - Intercalating Agents/*pharmacology
MH  - Kinetics
MH  - Microscopy, Fluorescence
MH  - Mutagens/pharmacology
MH  - Phosphodiesterase Inhibitors/pharmacology
MH  - Protein Binding
MH  - Spectrometry, Fluorescence
MH  - Time Factors
MH  - Uric Acid/*analogs & derivatives/pharmacology
MH  - Xanthine/pharmacology
EDAT- 2002/02/16 10:00
MHDA- 2002/05/22 10:01
CRDT- 2002/02/16 10:00
PHST- 2002/02/16 10:00 [pubmed]
PHST- 2002/05/22 10:01 [medline]
PHST- 2002/02/16 10:00 [entrez]
AID - S1065699501908108 [pii]
AID - 10.1006/cbir.2001.0810 [doi]
PST - ppublish
SO  - Cell Biol Int. 2002;26(2):145-54. doi: 10.1006/cbir.2001.0810.