PMID- 11846878
OWN - NLM
STAT- MEDLINE
DCOM- 20020604
LR  - 20240109
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 29
IP  - 4
DP  - 2002 Feb
TI  - The GAMYB protein from barley interacts with the DOF transcription factor BPBF 
      and activates endosperm-specific genes during seed development.
PG  - 453-64
AB  - HvGAMYB, a MYB transcription factor previously shown to be expressed in barley 
      aleurone cells in response to gibberellin during germination, also has an 
      important role in gene regulation during endosperm development. The mRNA was 
      detected early (10 DAF) in the seeds where it accumulates, not only in the 
      aleurone layer, starchy endosperm, nucellar projection and vascular tissue, but 
      also in the immature embryo as shown by in situ hybridization analysis. The 
      HvGAMYB protein, expressed in bacteria, binds to oligonucleotides containing the 
      5'-TAACAAC-3' or 5'-CAACTAAC-3' sequences, derived from the promoter regions of 
      the endosperm-specific genes Hor2 and Itr1, encoding a B-hordein and 
      trypsin-inhibitor BTI-CMe, respectively. Binding is prevented when these motifs 
      are mutated to 5'-TgACAAg-3' and 5'-CgACTgAC-3'. Transient expression experiments 
      in co-bombarded developing endosperms demonstrate that HvGAMYB trans-activates 
      transcription from native Hor2 and Itr1 promoters through binding to the intact 
      motifs described above. Trans-activation of the Hor2 promoter also requires an 
      intact prolamine box (PB). This suggests that HvGAMYB interacts in developing 
      barley endosperms with the PB-binding factor BPBF, an endosperm-specific DOF 
      transcriptional activator of the Hor2 gene. The in vivo interaction experiment 
      between HvGAMYB and BPBF was done in the yeast two-hybrid system, where HvGAMYB 
      potentiates the BPBF trans-activation capacity through interaction with its 
      C-terminal domain.
FAU - Diaz, Isabel
AU  - Diaz I
AD  - Laboratorio de Bioquimica y Biologia Molecular, Dpto. de Biotecnologia-UPM, ETS I 
      Agronomos, 28040 Madrid, Spain.
FAU - Vicente-Carbajosa, Jesus
AU  - Vicente-Carbajosa J
FAU - Abraham, Zamira
AU  - Abraham Z
FAU - Martinez, Manuel
AU  - Martinez M
FAU - Isabel-La Moneda, Ines
AU  - Isabel-La Moneda I
FAU - Carbonero, Pilar
AU  - Carbonero P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ITR protein, Hordeum vulgare)
RN  - 0 (Plant Proteins)
RN  - 0 (Proto-Oncogene Proteins c-myb)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 8002-80-0 (Glutens)
SB  - IM
MH  - Base Sequence
MH  - Binding Sites
MH  - Blotting, Northern
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Glutens
MH  - Hordeum/*genetics/growth & development
MH  - In Situ Hybridization
MH  - Plant Proteins/*genetics/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding
MH  - *Proto-Oncogene Proteins c-myb
MH  - RNA, Messenger/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Seeds/genetics/growth & development
MH  - Transcription Factors/*genetics/metabolism
MH  - Transcriptional Activation/genetics
MH  - Two-Hybrid System Techniques
EDAT- 2002/02/16 10:00
MHDA- 2002/06/05 10:01
CRDT- 2002/02/16 10:00
PHST- 2002/02/16 10:00 [pubmed]
PHST- 2002/06/05 10:01 [medline]
PHST- 2002/02/16 10:00 [entrez]
AID - 1230 [pii]
AID - 10.1046/j.0960-7412.2001.01230.x [doi]
PST - ppublish
SO  - Plant J. 2002 Feb;29(4):453-64. doi: 10.1046/j.0960-7412.2001.01230.x.