PMID- 11847588
OWN - NLM
STAT- MEDLINE
DCOM- 20020417
LR  - 20181113
IS  - 1355-0284 (Print)
IS  - 1355-0284 (Linking)
VI  - 8
IP  - 1
DP  - 2002 Feb
TI  - Expression of brain-derived neurotrophic factor in the central nervous system of 
      mice using a poliovirus-based vector.
PG  - 14-23
AB  - Brain-derived neurotrophic factor (BDNF) is a promising candidate for the gene 
      therapy of neurological disease. To deliver BDNF to neurons of the central 
      nervous system (CNS), a nucleotide sequence encoding the mature peptide of BDNF 
      was inserted into the genome of poliovirus, a neurotropic virus that is known to 
      replicate mainly in motor neurons of the spinal cord of the CNS. Thus, the 
      recombinant poliovirus constructed was replication-competent. The expression of 
      BDNF in cultured cells infected with the recombinant poliovirus was evident when 
      the cells were analyzed using an immunofluorescence assay and Western blotting. 
      When the recombinant viruses were injected intramuscularly into transgenic mice 
      that carry the human poliovirus receptor gene, the antigens of poliovirus and 
      BDNF were detected in the motor neurons of the spinal cord at 3 days 
      postinfection, and had disappeared by 7 days postinfection. This study suggests 
      that poliovirus can be used as a virus vector for the delivery of neurotrophic 
      factors to the motor neurons of the central nervous system and may provide a new 
      approach for the treatment of motor neuron diseases.
FAU - Jia, Qingmei
AU  - Jia Q
AD  - Department of Microbiology, Graduate School of Medicine, The University of Tokyo, 
      Tokyo, Japan.
FAU - Liang, Fengyi
AU  - Liang F
FAU - Ohka, Seii
AU  - Ohka S
FAU - Nomoto, Akio
AU  - Nomoto A
FAU - Hashikawa, Tsutomu
AU  - Hashikawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Virus)
RN  - 0 (poliovirus receptor)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cells, Cultured
MH  - Central Nervous System/cytology/*virology
MH  - Gene Expression Regulation, Viral
MH  - *Genetic Vectors
MH  - Injections, Intramuscular
MH  - *Membrane Proteins
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Motor Neurons/virology
MH  - Poliovirus/*genetics/growth & development
MH  - Receptors, Virus/genetics
MH  - Virus Replication
EDAT- 2002/02/16 10:00
MHDA- 2002/04/18 10:01
CRDT- 2002/02/16 10:00
PHST- 2002/02/16 10:00 [pubmed]
PHST- 2002/04/18 10:01 [medline]
PHST- 2002/02/16 10:00 [entrez]
AID - 10.1080/135502802317247776 [doi]
PST - ppublish
SO  - J Neurovirol. 2002 Feb;8(1):14-23. doi: 10.1080/135502802317247776.