PMID- 11847589
OWN - NLM
STAT- MEDLINE
DCOM- 20020417
LR  - 20200327
IS  - 1355-0284 (Print)
IS  - 1538-2443 (Electronic)
IS  - 1355-0284 (Linking)
VI  - 8
IP  - 1
DP  - 2002 Feb
TI  - Infection of human oligodendroglioma cells by a recombinant measles virus 
      expressing enhanced green fluorescent protein.
PG  - 24-34
AB  - One of the hallmarks of the human CNS disease subacute sclerosing panencephalitis 
      (SSPE) is a high level of measles virus (MV) infection of oligodendrocytes. It is 
      therefore surprising that there is only one previous report of MV infection of 
      rat oligodendrocytes in culture and no reports of human oligodendrocyte infection 
      in culture. In an attempt to develop a model system to study MV infection of 
      oligodendrocytes, time-lapse confocal microscopy, immunocytochemistry, and 
      electron microscopy (EM) were used to study infection of the human 
      oligodendroglioma cell line, MO3.13. A rat oligodendrocyte cell line, OLN-93, was 
      also studied as a control. MO3.13 cells were shown to be highly susceptible to MV 
      infection and virus budding was observed from the surface of infected MO3.13 
      cells by EM. Analysis of the infection in real time and by immunocytochemistry 
      revealed that virus spread occurred by cell-to-cell fusion and was also 
      facilitated by virus transport in cell processes. MO3.13 cells were shown to 
      express CD46, a MV receptor, but were negative for the recently discovered MV 
      receptor, signaling leucocyte activation molecule (SLAM). Immunohistochemical 
      studies on SSPE tissue sections demonstrated that CD46 was also expressed on 
      populations of human oligodendrocytes. SLAM expression was not detected on 
      oligodendrocytes. These studies, which are the first to show MV infection of 
      human oligodendrocytes in culture, show that the cells are highly susceptible to 
      MV infection and this model cell line has been used to further our understanding 
      of MV spread in the CNS.
FAU - Plumb, Jonnie
AU  - Plumb J
AD  - Neuropathology Laboratory, Royal Group of Hospitals Trust, Belfast, Northern 
      Ireland, United Kingdom.
FAU - Duprex, W Paul
AU  - Duprex WP
FAU - Cameron, C H Stewart
AU  - Cameron CH
FAU - Richter-Landsberg, Christiane
AU  - Richter-Landsberg C
FAU - Talbot, Pierre
AU  - Talbot P
FAU - McQuaid, Stephen
AU  - McQuaid S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurovirol
JT  - Journal of neurovirology
JID - 9508123
RN  - 0 (Indicators and Reagents)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Indicators and Reagents/metabolism
MH  - Luminescent Proteins/genetics
MH  - Measles virus/*genetics/growth & development
MH  - Oligodendroglia/cytology/*virology
MH  - *Oligodendroglioma
MH  - Rats
MH  - Recombinant Proteins/genetics
MH  - Subacute Sclerosing Panencephalitis/*virology
MH  - Tumor Cells, Cultured/virology
PMC - PMC7095342
EDAT- 2002/02/16 10:00
MHDA- 2002/04/18 10:01
PMCR- 2020/03/25
CRDT- 2002/02/16 10:00
PHST- 2002/02/16 10:00 [pubmed]
PHST- 2002/04/18 10:01 [medline]
PHST- 2002/02/16 10:00 [entrez]
PHST- 2020/03/25 00:00 [pmc-release]
AID - 80100024 [pii]
AID - 10.1080/135502802317247785 [doi]
PST - ppublish
SO  - J Neurovirol. 2002 Feb;8(1):24-34. doi: 10.1080/135502802317247785.