PMID- 11848469
OWN - NLM
STAT- MEDLINE
DCOM- 20020312
LR  - 20061115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 21
IP  - 5
DP  - 2001 Sep-Oct
TI  - Chromosome 8 numerical aberration and C-MYC copy number gain in bladder cancer 
      are linked to stage and grade.
PG  - 3167-73
AB  - Chromosome 8 aberration and c-myc amplification have been suggested as playing 
      important roles in the development of different human cancers. Using fluorescence 
      in situ hybridization (FISH), chromosome 8 polysomy and c-myc amplification can 
      be detected in cells from bladder cancer. We investigated the correlation of 
      chromosome 8 polysomy, c-myc gene alteration and p53 deletion with 
      histopathological parameters. Twenty-four tumors obtained from patients with 
      bladder cancer were analyzed by interphase cytogenetics using FISH with 
      chromosome 8 and 17 centromere probes together with an YAC clone covering the 
      c-myc locus and three cosmid DNA probes covering the p53 locus. Chromosome 8 
      polysomy was found in 12 tumors. The average copy number of chromosome 8 
      centromere signals were significantly higher in high grade and stage, cancers. 
      Also the c-myc copy gain and p53 deletion were significantly correlated with 
      grade as well as stage (p<0.05, in both cases). Both polysomy 8 and c-myc copy 
      gain were significantly correlated with p53 deletions (p<0.01) and DNA ploidy 
      (p<0.001). On the contrary there was no significant correlation between c-myc 
      protein over-expression and c-myc gene amplification. These results may indicate 
      that alteration of chromosomal regions on 8q and 17p, including c-myc and p53 
      genes, may be linked to progression of bladder cancer.
FAU - Mahdy, E
AU  - Mahdy E
AD  - Department of Urology, Karolinska Hospital, Stockholm, Sweden. 
      Ensaf.Mahdy@kirurgi.ki.se
FAU - Pan, Y
AU  - Pan Y
FAU - Wang, N
AU  - Wang N
FAU - Malmstrom, P U
AU  - Malmstrom PU
FAU - Ekman, P
AU  - Ekman P
FAU - Bergerheim, U
AU  - Bergerheim U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
MH  - Carcinoma, Transitional Cell/*genetics/metabolism/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 8
MH  - DNA, Neoplasm/genetics
MH  - Gene Dosage
MH  - *Genes, myc
MH  - Genes, p53
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Neoplasm Staging
MH  - Ploidies
MH  - Proto-Oncogene Proteins c-myc/biosynthesis/genetics
MH  - Urinary Bladder Neoplasms/*genetics/metabolism/pathology
EDAT- 2002/02/19 10:00
MHDA- 2002/03/13 10:01
CRDT- 2002/02/19 10:00
PHST- 2002/02/19 10:00 [pubmed]
PHST- 2002/03/13 10:01 [medline]
PHST- 2002/02/19 10:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2001 Sep-Oct;21(5):3167-73.