PMID- 11849317
OWN - NLM
STAT- MEDLINE
DCOM- 20020304
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 105
IP  - 1
DP  - 2002 Jan
TI  - Expression of matrix metalloproteinases and tissue inhibitors of 
      metalloproteinases define the migratory characteristics of human monocyte-derived 
      dendritic cells.
PG  - 73-82
AB  - Dendritic cells (DCs) have an essential role in the initiation of immune 
      responses as they deliver antigen/epitope and the appropriate signals to activate 
      naive T cells and thus start an immune response. In order to fulfil their 
      function, DCs have to patrol different part of the body, thus migrating through 
      the extracellular matrix to sample the local 'antigenic' environment. In the 
      present study, we have investigated which enzymes might be involved in this 
      process using the Matrigel trans-well migration assay, an in vitro model of 
      extracellular matrix migration. In this assay we analysed the migratory ability 
      of interleukin-4 (IL-4)/granulocyte macrophage-colony-stimulating factor 
      (GM-CSF)-derived immature DCs as well as mature DCs, induced by tumour necrosis 
      factor-alpha (TNF-alpha) and modified vaccinia virus Ankara (MVA). The 'mature' 
      DCs showed an increased migration through Matrigel, which was significantly 
      inhibited by inhibitors of matrix metalloproteinases (MMP). We also observed that 
      the dominant MMP involved in this process was MMP-9, and a concomitant decrease 
      of the endogenous tissue inhibitors of metalloproteinases (TIMP)-1 and TIMP-2 was 
      also observed. Collectively these data suggest that the balance between MMP/TIMP 
      determines the net migratory capacity of human DCs. Surprisingly, TIMP-3 was 
      significantly increased in mature DC. Our data thus indicate that MMP and TIMP 
      play a role in the migratory ability of human DCs. Our results also suggest that 
      TIMP-3 expression might represent a new marker of maturation of human DCs.
FAU - Osman, Mohamed
AU  - Osman M
AD  - Imperial College School of Medicine, Kennedy Institute of Rheumatology Division, 
      London, UK. m.osman@ic.ac.uk
FAU - Tortorella, Micky
AU  - Tortorella M
FAU - Londei, Marco
AU  - Londei M
FAU - Quaratino, Sonia
AU  - Quaratino S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Blotting, Western
MH  - Cell Migration Inhibition
MH  - Cell Movement/*physiology
MH  - Dendritic Cells/cytology/*physiology
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/physiology
MH  - Humans
MH  - Interleukin-4/physiology
MH  - Matrix Metalloproteinases/*physiology
MH  - Monocytes/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Inhibitor of Metalloproteinases/*physiology
MH  - Tumor Necrosis Factor-alpha/physiology
MH  - Vaccinia virus/physiology
PMC - PMC1782644
EDAT- 2002/02/19 10:00
MHDA- 2002/03/05 10:01
PMCR- 2003/01/01
CRDT- 2002/02/19 10:00
PHST- 2002/02/19 10:00 [pubmed]
PHST- 2002/03/05 10:01 [medline]
PHST- 2002/02/19 10:00 [entrez]
PHST- 2003/01/01 00:00 [pmc-release]
AID - 1349 [pii]
AID - 10.1046/j.0019-2805.2001.01349.x [doi]
PST - ppublish
SO  - Immunology. 2002 Jan;105(1):73-82. doi: 10.1046/j.0019-2805.2001.01349.x.