PMID- 11850072 OWN - NLM STAT- MEDLINE DCOM- 20020320 LR - 20190816 IS - 0165-4608 (Print) IS - 0165-4608 (Linking) VI - 132 IP - 2 DP - 2002 Jan 15 TI - A comprehensive karyotypic analysis on a newly established sarcomatoid hepatocellular carcinoma cell line SH-J1 by comparative genomic hybridization and chromosome painting. PG - 120-4 AB - We first established a sarcomatoid hepatocellular carcinoma cell line, designated as SH-J1, and applied comparative genomic hybridization and fluorescence in situ hybridization (FISH) with chromosome painting probes for the characterization of the chromosomal rearrangements. In the SH-J1 cell line, the pleomorphic spindle cells were arranged in bundles of interlacing patterns and were positive in immunohistochemical staining with hepatocyte-related markers. By G-banding and FISH, the chromosomal gains were detected at 6p and 17, whereas losses were observed at 3p21-pter, 3q27-qter, 4, 6q, 13pter-q11, 16, 18, 19p13, and Y. FAU - Kim, Dae Ghon AU - Kim DG AD - Research Institute of Clinical Medicine, Department of Internal Medicine, Chonbuk National University Medical School, Chonju, South Korea. FAU - Park, Soo Yeun AU - Park SY FAU - Kim, Hyun AU - Kim H FAU - Chun, Yong Hyuck AU - Chun YH FAU - Moon, Woo Sung AU - Moon WS FAU - Park, Sun Hwa AU - Park SH LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cancer Genet Cytogenet JT - Cancer genetics and cytogenetics JID - 7909240 SB - IM MH - Carcinoma, Hepatocellular/*genetics/pathology MH - Chromosome Banding MH - *Chromosome Painting MH - Humans MH - Karyotyping MH - Liver Neoplasms/*genetics/pathology MH - Male MH - Middle Aged MH - Nucleic Acid Hybridization/*methods MH - Tumor Cells, Cultured EDAT- 2002/02/19 10:00 MHDA- 2002/03/21 10:01 CRDT- 2002/02/19 10:00 PHST- 2002/02/19 10:00 [pubmed] PHST- 2002/03/21 10:01 [medline] PHST- 2002/02/19 10:00 [entrez] AID - S016546080100543X [pii] AID - 10.1016/s0165-4608(01)00543-x [doi] PST - ppublish SO - Cancer Genet Cytogenet. 2002 Jan 15;132(2):120-4. doi: 10.1016/s0165-4608(01)00543-x.