PMID- 11850076
OWN - NLM
STAT- MEDLINE
DCOM- 20020320
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 132
IP  - 2
DP  - 2002 Jan 15
TI  - Fluorescence in situ hybridization analysis of HER-2/neu in brushings of normal 
      oral mucosa.
PG  - 141-4
AB  - Oncogene alterations have been clearly demonstrated to be related to the 
      carcinogenesis and progression of oral squamous cell carcinoma (OSCC). However, 
      the analysis of these alterations for screening and early diagnostic purposes 
      generally requires invasive techniques for surgical removal of pathological 
      epithelium. The aim of the present study was to assess the feasibility of 
      fluorescence in situ hybridization (FISH) analysis of HER-2/neu amplification in 
      oral mucosa brushings and to compare the HER-2/neu status with the history and 
      smoking and drinking habits of healthy subjects. Cells obtained by centrifugation 
      of oral brushings from 21 subjects (overall no. of cells: 5125) were suspended in 
      physiological saline and fixed onto two slides for cytological evaluation and 
      FISH analysis (dual-target, dual-color fluorescence assay) of the HER-2/neu gene 
      and CEP17 centromere. A mean of 89.8% of the cells showed two HER-2/neu signals 
      and a mean of 94% had two CEP17 signals at fluorescent microscopy. Finally, a 
      mean of 96% of cells with HER-2/neu / CEP17 had a ratio equal to 1. No 
      association between smoking and drinking habits, age and the HER-2/neu and CEP17 
      characteristics evaluated by FISH was found.
FAU - Paradiso, Angelo
AU  - Paradiso A
AD  - Clinical Experimental Oncology Laboratory, National Cancer Institute, Via 
      Amendola 209, Bari, Italy. anpara@tin.it
FAU - Abatangelo, Marta
AU  - Abatangelo M
FAU - Piepoli, Sandra
AU  - Piepoli S
FAU - Tommasi, Stefania
AU  - Tommasi S
FAU - Xu, Jian Ming
AU  - Xu JM
FAU - Caponio, Maria Angela
AU  - Caponio MA
FAU - Marzullo, Franco
AU  - Marzullo F
FAU - D'Auria, Carlo
AU  - D'Auria C
FAU - Achille, Gaetano
AU  - Achille G
FAU - Grammatica, Luciano
AU  - Grammatica L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - Mouth Mucosa/*metabolism
MH  - Receptor, ErbB-2/*genetics
EDAT- 2002/02/19 10:00
MHDA- 2002/03/21 10:01
CRDT- 2002/02/19 10:00
PHST- 2002/02/19 10:00 [pubmed]
PHST- 2002/03/21 10:01 [medline]
PHST- 2002/02/19 10:00 [entrez]
AID - S0165460801005374 [pii]
AID - 10.1016/s0165-4608(01)00537-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Jan 15;132(2):141-4. doi: 
      10.1016/s0165-4608(01)00537-4.