PMID- 11850459
OWN - NLM
STAT- MEDLINE
DCOM- 20020226
LR  - 20191023
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 22
IP  - 4
DP  - 2002 Feb 15
TI  - Brain-derived neurotrophic factor modulates cerebellar plasticity and synaptic 
      ultrastructure.
PG  - 1316-27
AB  - Neurotrophins are key regulators of neuronal survival and function. Here we show 
      that TrkB, the receptor for brain-derived neurotrophic factor (BDNF), is located 
      at parallel fiber to Purkinje cell (PF/PC) synapses of the cerebellum. To 
      determine the effects of TrkB receptor activation on synapse formation and 
      function, we examined the parallel fiber to Purkinje cell synapses of mice with a 
      targeted deletion of the BDNF gene. Although Purkinje cell dendrites are abnormal 
      in BDNF -/- mice, PF/PC synapses are still able to form. Immunohistochemical 
      analysis of mutant animals revealed the formation of numerous PF/PC synapses with 
      the appropriate apposition of presynaptic and postsynaptic proteins. These 
      synapses are functional, and no differences were detected in the waveform of 
      evoked EPSCs, the amplitude of spontaneous mini-EPSCs, or the response to 
      prolonged 10 Hz stimulus trains. However, paired-pulse facilitation, a form of 
      short-term plasticity, is significantly decreased in BDNF -/- mice. Detailed 
      ultrastructural analysis of the presynaptic terminals demonstrated that this 
      change in synaptic function is accompanied by an increase in the total number of 
      synaptic vesicles in mutant mice and a decrease in the proportion of vesicles 
      that are docked. These data suggest that BDNF regulates both the mechanisms that 
      underlie short-term synaptic plasticity and the steady-state relationship between 
      different vesicle pools within the terminal.
FAU - Carter, Alexandre R
AU  - Carter AR
AD  - Department of Pediatric Oncology, Dana Farber Cancer Institute, Harvard Medical 
      School, Boston, Massachusetts 02115, USA.
FAU - Chen, Chinfei
AU  - Chen C
FAU - Schwartz, Phillip M
AU  - Schwartz PM
FAU - Segal, Rosalind A
AU  - Segal RA
LA  - eng
GR  - F31 LM000040/LM/NLM NIH HHS/United States
GR  - R01 NS037757/NS/NINDS NIH HHS/United States
GR  - NS37757/NS/NINDS NIH HHS/United States
GR  - 5F31LM00040-05/LM/NLM NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calbindins)
RN  - 0 (Receptors, Glutamate)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 0 (glutamate receptor delta 2)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/deficiency/genetics/*metabolism/pharmacology
MH  - Calbindins
MH  - Cerebellum/cytology/*metabolism
MH  - Dendrites/ultrastructure
MH  - Electric Stimulation
MH  - Excitatory Postsynaptic Potentials/physiology
MH  - Gene Deletion
MH  - In Vitro Techniques
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Mutant Strains
MH  - Neuronal Plasticity/drug effects/*physiology
MH  - Patch-Clamp Techniques
MH  - Presynaptic Terminals/drug effects/metabolism/ultrastructure
MH  - Purkinje Cells/metabolism/ultrastructure
MH  - Receptor, trkB/metabolism
MH  - Receptors, Glutamate/metabolism
MH  - S100 Calcium Binding Protein G/metabolism
MH  - Synapses/drug effects/metabolism/*ultrastructure
MH  - Synaptic Transmission/physiology
MH  - Synaptic Vesicles/ultrastructure
PMC - PMC6757568
EDAT- 2002/02/19 10:00
MHDA- 2002/02/28 10:01
PMCR- 2002/08/15
CRDT- 2002/02/19 10:00
PHST- 2002/02/19 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2002/02/19 10:00 [entrez]
PHST- 2002/08/15 00:00 [pmc-release]
AID - 22/4/1316 [pii]
AID - 6081 [pii]
AID - 10.1523/JNEUROSCI.22-04-01316.2002 [doi]
PST - ppublish
SO  - J Neurosci. 2002 Feb 15;22(4):1316-27. doi: 10.1523/JNEUROSCI.22-04-01316.2002.