PMID- 11852185
OWN - NLM
STAT- MEDLINE
DCOM- 20020425
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 320
IP  - 3
DP  - 2002 Mar 8
TI  - Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid 
      of Alzheimer disease patients.
PG  - 156-60
AB  - Neurofilament (NF) subunits NF-H, NF-M and NF-L are hyperphosphorylated and 
      elevated in Alzheimer disease (AD) brain. We investigated the level and 
      phosphorylation states of NF subunits in lumbar cerebrospinal fluid (CSF) from 
      living patients by bienzyme substrate-recycle enzyme-linked immunosorbent assay. 
      We found: (i), that the levels of phosphorylated NF-H/M (pNF-H/M), 
      non-phosphorylated NF-H/M (npNF-H/M) and NF-L were significantly higher (pNF-H/M, 
      approximately 12-24-fold; npNF-H/M, approximately 3-4-fold) in neurologically 
      healthy aged people than young control individuals; (ii), that in AD, the levels 
      of npNF-H/M, and NF-L were similar to vascular dementia (VaD), and higher than in 
      age-matched controls; and (iii), that the levels of pNF-H/M were significantly 
      higher than in aged controls, non-AD neurological disorders and VaD. Based on 
      these findings, it is suggested that the increased level of total NF proteins in 
      CSF could be used as a marker for brain aging and neurodegenerative disorders in 
      general, and the levels of pNF-H/M as a marker to discriminate AD from normal 
      brain aging and as well as neurological conditions including VaD.
FAU - Hu, Yuan-Yuan
AU  - Hu YY
AD  - Tongji Medical College, HUST, Wuhan 430030, PR China.
FAU - He, Shan-Shu
AU  - He SS
FAU - Wang, Xiao-Chuang
AU  - Wang XC
FAU - Duan, Qiu-Hong
AU  - Duan QH
FAU - Khatoon, Sabiha
AU  - Khatoon S
FAU - Iqbal, Khalid
AU  - Iqbal K
FAU - Grundke-Iqbal, Inge
AU  - Grundke-Iqbal I
FAU - Wang, Jian-Zhi
AU  - Wang JZ
LA  - eng
GR  - TWOO703/TW/FIC NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Neurofilament Proteins)
RN  - 0 (neurofilament protein L)
RN  - 0 (tau Proteins)
RN  - 108688-71-7 (neurofilament protein H)
RN  - 111365-29-8 (neurofilament protein M)
SB  - IM
MH  - Adolescent
MH  - Age Factors
MH  - Aged
MH  - Alzheimer Disease/*cerebrospinal fluid
MH  - Brain/*metabolism/pathology/physiopathology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Immunoassay
MH  - Male
MH  - Middle Aged
MH  - Neurofilament Proteins/*cerebrospinal fluid
MH  - Neurons/*metabolism/pathology
MH  - Phosphorylation
MH  - Up-Regulation/*physiology
MH  - tau Proteins/cerebrospinal fluid
EDAT- 2002/02/20 10:00
MHDA- 2002/04/26 10:01
CRDT- 2002/02/20 10:00
PHST- 2002/02/20 10:00 [pubmed]
PHST- 2002/04/26 10:01 [medline]
PHST- 2002/02/20 10:00 [entrez]
AID - S0304394002000472 [pii]
AID - 10.1016/s0304-3940(02)00047-2 [doi]
PST - ppublish
SO  - Neurosci Lett. 2002 Mar 8;320(3):156-60. doi: 10.1016/s0304-3940(02)00047-2.