PMID- 11853317
OWN - NLM
STAT- MEDLINE
DCOM- 20020812
LR  - 20240109
IS  - 1340-2838 (Print)
IS  - 1340-2838 (Linking)
VI  - 8
IP  - 6
DP  - 2001 Dec 31
TI  - Construction of a genetic linkage map of the model legume Lotus japonicus using 
      an intraspecific F2 population.
PG  - 301-10
AB  - Among leguminous plants, the model legume Lotus japonicus (Regel) Larsen has many 
      biological and genetic advantages. We have developed a genetic linkage map of L. 
      japonicus based on amplified fragment length polymorphism (AFLP), simple sequence 
      repeat polymorphism (SSRP) and derived cleaved amplified polymorphic sequence 
      (dCAPS). The F2 mapping population used was derived from a cross between two L. 
      japonicus accessions Gifu B-129 and Miyakojima MG-20. These parental accessions 
      showed remarkable cytological differences, particularly with respect to size and 
      morphology of chromosomes 1 and 2. Using fluorescence in situ hybridization 
      (FISH) with BAC clones from Gifu B-129 and TAC (Transformation-competent 
      Artificial Chromosome) clones from Miyakojima MG-20, a reciprocal translocation 
      was found to be responsible for the cytological differences between chromosomes 1 
      and 2. The borders of the translocations were identified by FISH and by alignment 
      toward the L. filicaulis x L. japonicus Gifu B-129 linkage map. The markers from 
      the main translocated region were located on linkage groups 1 and 2 of the two 
      accessions, Gifu B-129 and Miyakojima MG-20, respectively. The framework of the 
      linkage map was constructed based on codominant markers, and then dominant 
      markers were integrated separately in each linkage group of the parents. The 
      resulting linkage groups correspond to the six pairs of chromosomes of L. 
      japonicus and consist of 287 markers with 487.3 cM length in Gifu B-129 and 277 
      markers with 481.6 cM length in Miyakojima MG-20. The map and marker information 
      is available through the World Wide Web at http://www.kazusa.or.jp/lotus/.
FAU - Hayashi, M
AU  - Hayashi M
AD  - Faculty of Horticulture, Chiba University, Matsudo, Japan.
FAU - Miyahara, A
AU  - Miyahara A
FAU - Sato, S
AU  - Sato S
FAU - Kato, T
AU  - Kato T
FAU - Yoshikawa, M
AU  - Yoshikawa M
FAU - Taketa, M
AU  - Taketa M
FAU - Hayashi, M
AU  - Hayashi M
FAU - Pedrosa, A
AU  - Pedrosa A
FAU - Onda, R
AU  - Onda R
FAU - Imaizumi-Anraku, H
AU  - Imaizumi-Anraku H
FAU - Bachmair, A
AU  - Bachmair A
FAU - Sandal, N
AU  - Sandal N
FAU - Stougaard, J
AU  - Stougaard J
FAU - Murooka, Y
AU  - Murooka Y
FAU - Tabata, S
AU  - Tabata S
FAU - Kawasaki, S
AU  - Kawasaki S
FAU - Kawaguchi, M
AU  - Kawaguchi M
FAU - Harada, K
AU  - Harada K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes 
      and genomes
JID - 9423827
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Mapping
MH  - Genes, Plant/*physiology
MH  - *Genetic Linkage
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Lotus/*genetics/growth & development
MH  - Mitosis
MH  - Models, Biological
MH  - Plant Structures
MH  - Polymorphism, Restriction Fragment Length
MH  - Random Amplified Polymorphic DNA Technique
EDAT- 2002/02/21 10:00
MHDA- 2002/08/13 10:01
CRDT- 2002/02/21 10:00
PHST- 2002/02/21 10:00 [pubmed]
PHST- 2002/08/13 10:01 [medline]
PHST- 2002/02/21 10:00 [entrez]
AID - 10.1093/dnares/8.6.301 [doi]
PST - ppublish
SO  - DNA Res. 2001 Dec 31;8(6):301-10. doi: 10.1093/dnares/8.6.301.