PMID- 11854206
OWN - NLM
STAT- MEDLINE
DCOM- 20020401
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 70
IP  - 3
DP  - 2002 Mar
TI  - Recombinant Dirofilaria immitis polyprotein that stimulates murine B cells to 
      produce nonspecific polyclonal immunoglobulin E antibody.
PG  - 1235-44
AB  - Nonspecific immunoglobulin E (IgE) production is an event characteristically 
      observed in parasitic helminth infections, but its mechanisms are still unclear. 
      To define these mechanisms, we prepared a recombinant Dirofilaria immitis protein 
      (rDiAg) and assessed its effect on nonspecific IgE production. rDiAg 
      preferentially induced nonspecific IgE production, without eliciting specific IgE 
      production, as well as a Th2-type cytokine profile (high interleukin-4 [IL-4] and 
      IL-10 production but low gamma interferon production) in BALB/c mice. rDiAg 
      significantly elicited the proliferative response of naive B cells. This response 
      was not abolished by polymyxin B, an inhibitor of lipopolysaccharide (LPS), and 
      rDiAg normally expanded splenic B cells from LPS nonresponder C3H/HeJ mice. Thus, 
      the mitogenic effect of rDiAg was not due to LPS contamination. rDiAg also 
      enhanced levels of CD23 expression on splenic B cells. Splenic B cells produced 
      marked levels of IgE when cultured with the combination of rDiAg and IL-4 
      (rDiAg-IL-4), whereas peritoneal B cells produced negligible levels of IgE. 
      rDiAg-IL-4-induced IgE production by splenic B cells was synergistically 
      increased by coculture with peritoneal B cells. rDiAg-driven IL-10 secretion was 
      higher in peritoneal B cells than in splenic B cells. IgE production by splenic B 
      cells cocultured with peritoneal B cells was decreased to a level comparable to 
      that by splenic B cells in the presence of a neutralizing anti-IL-10 monoclonal 
      antibody. Collectively, these results suggest that rDiAg-induced polyclonal 
      expansion and IgE class switching of splenic B cells contribute to nonspecific 
      IgE production and that these responses are enhanced by peritoneal B-cell-derived 
      IL-10.
FAU - Tezuka, Hiroyuki
AU  - Tezuka H
AD  - Section of Environmental Parasitology, Department of International Health 
      Development, Division of Public Health, Graduate School, Tokyo Medical and Dental 
      University, 1-5-45 Yushima Bunkyo-ku, Tokyo 113-8519, Japan.
FAU - Imai, Shinjiro
AU  - Imai S
FAU - Muto, Riho
AU  - Muto R
FAU - Furuhashi, Yuko
AU  - Furuhashi Y
FAU - Fujita, Koichiro
AU  - Fujita K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Helminth)
RN  - 0 (Antigens, Helminth)
RN  - 0 (DIPA protein, Dirofilaria immitis)
RN  - 0 (Helminth Proteins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Helminth/*blood
MH  - Antibody Specificity
MH  - Antigens, Helminth/*immunology
MH  - B-Lymphocytes/*immunology
MH  - Cell Differentiation
MH  - Dirofilaria immitis/*immunology
MH  - Female
MH  - Helminth Proteins/*immunology
MH  - Immunoglobulin Class Switching
MH  - Immunoglobulin E/*blood
MH  - Interferon-gamma/blood
MH  - Interleukin-10/blood
MH  - Interleukin-4/blood
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peritoneal Cavity/cytology
MH  - Rabbits
MH  - Spleen/cytology
MH  - Th2 Cells
PMC - PMC127741
EDAT- 2002/02/21 10:00
MHDA- 2002/04/02 10:01
PMCR- 2002/03/01
CRDT- 2002/02/21 10:00
PHST- 2002/02/21 10:00 [pubmed]
PHST- 2002/04/02 10:01 [medline]
PHST- 2002/02/21 10:00 [entrez]
PHST- 2002/03/01 00:00 [pmc-release]
AID - 0772 [pii]
AID - 10.1128/IAI.70.3.1235-1244.2002 [doi]
PST - ppublish
SO  - Infect Immun. 2002 Mar;70(3):1235-44. doi: 10.1128/IAI.70.3.1235-1244.2002.