PMID- 11854360
OWN - NLM
STAT- MEDLINE
DCOM- 20020321
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 195
IP  - 4
DP  - 2002 Feb 18
TI  - Complementary dendritic cell-activating function of CD8+ and CD4+ T cells: helper 
      role of CD8+ T cells in the development of T helper type 1 responses.
PG  - 473-83
AB  - Dendritic cells (DCs) activated by CD40L-expressing CD4+ T cells act as mediators 
      of "T helper (Th)" signals for CD8+ T lymphocytes, inducing their cytotoxic 
      function and supporting their long-term activity. Here, we show that the optimal 
      activation of DCs, their ability to produce high levels of bioactive interleukin 
      (IL)-12p70 and to induce Th1-type CD4+ T cells, is supported by the complementary 
      DC-activating signals from both CD4+ and CD8+ T cells. Cord blood- or peripheral 
      blood-isolated naive CD8+ T cells do not express CD40L, but, in contrast to naive 
      CD4+ T cells, they are efficient producers of IFN-gamma at the earliest stages of 
      the interaction with DCs. Naive CD8+ T cells cooperate with CD40L-expressing 
      naive CD4+ T cells in the induction of IL-12p70 in DCs, promoting the development 
      of primary Th1-type CD4+ T cell responses. Moreover, the recognition of major 
      histocompatibility complex class I-presented epitopes by antigen-specific CD8+ T 
      cells results in the TNF-alpha- and IFN-gamma-dependent increase in the 
      activation level of DCs and in the induction of type-1 polarized mature DCs 
      capable of producing high levels of IL-12p70 upon a subsequent CD40 ligation. The 
      ability of class I-restricted CD8+ T cells to coactivate and polarize DCs may 
      support the induction of Th1-type responses against class I-presented epitopes of 
      intracellular pathogens and contact allergens, and may have therapeutical 
      implications in cancer and chronic infections.
FAU - Mailliard, Robbie B
AU  - Mailliard RB
AD  - Department of Surgery, Cancer Institute, University of Pittsburgh, 200 Lothrop 
      Street, Pittsburgh, PA 15261, USA.
FAU - Egawa, Shinichi
AU  - Egawa S
FAU - Cai, Quan
AU  - Cai Q
FAU - Kalinska, Anna
AU  - Kalinska A
FAU - Bykovskaya, Svetlana N
AU  - Bykovskaya SN
FAU - Lotze, Michael T
AU  - Lotze MT
FAU - Kapsenberg, Martien L
AU  - Kapsenberg ML
FAU - Storkus, Walter J
AU  - Storkus WJ
FAU - Kalinski, Pawel
AU  - Kalinski P
LA  - eng
GR  - R01 CA057840/CA/NCI NIH HHS/United States
GR  - 1P01 CA 74343/CA/NCI NIH HHS/United States
GR  - 1R01 CA 57840/CA/NCI NIH HHS/United States
GR  - 1R01 CA 82016/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - CD40 Ligand/biosynthesis/immunology
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Influenza, Human/immunology
MH  - Interferon-gamma/biosynthesis/pharmacology
MH  - Interleukin-12/biosynthesis/immunology
MH  - Melanoma/immunology
MH  - Th1 Cells/*immunology/metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis/immunology
PMC - PMC2193623
EDAT- 2002/02/21 10:00
MHDA- 2002/03/22 10:01
PMCR- 2002/08/18
CRDT- 2002/02/21 10:00
PHST- 2002/02/21 10:00 [pubmed]
PHST- 2002/03/22 10:01 [medline]
PHST- 2002/02/21 10:00 [entrez]
PHST- 2002/08/18 00:00 [pmc-release]
AID - 011662 [pii]
AID - 10.1084/jem.20011662 [doi]
PST - ppublish
SO  - J Exp Med. 2002 Feb 18;195(4):473-83. doi: 10.1084/jem.20011662.