PMID- 11854595
OWN - NLM
STAT- MEDLINE
DCOM- 20020425
LR  - 20191105
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 11
IP  - 1
DP  - 2002 Mar
TI  - Immunoglobulin gene rearrangement analysis in composite hodgkin disease and large 
      B-cell lymphoma: evidence for receptor revision of immunoglobulin heavy chain 
      variable region genes in Hodgkin-Reed-Sternberg cells?
PG  - 2-8
AB  - Immunoglobulin heavy chain gene (IgH) rearrangement was studied in a patient 
      showing the occurrence of classical Hodgkin disease and large B-cell lymphoma 
      (LBCL) in the same lymph node. The VHDHJH region was amplified by polymerase 
      chain reaction, the template being the DNA extracted from single Hodgkin and 
      Reed-Sternberg and LBCL cells, microdissected on hematoxylin-eosin-stained 
      sections by laser capture. A repeated VH4DH3JH4 segment was found in 
      Reed-Sternberg cells, whereas a repeated VH3DH3JH4 segment was observed in LBCL 
      cells. Rearranged VH genes carried somatic mutations in both populations, 
      indicating a common germinal center cell origin. The IgH rearrangement found in 
      clonally related Reed-Sternberg cells differed from the one of LBCL cells in the 
      VH region but showed the same JH and DH segments with no variation from the 
      respective germline sequence. The DH-JH junction is the first immunoglobulin gene 
      segment rearranged in precursor B cells. Because the possibility of secondary Ig 
      gene rearrangement in peripheral lymphoid organs has recently been reported, in 
      the patient described here Reed-Sternberg and LBCL cells might originate from a 
      common precursor in which secondary VH replacement took place during the germinal 
      center reaction, giving rise to two different clonally related lymphomas.
FAU - Bellan, Cristiana
AU  - Bellan C
AD  - Institute of Pathologic Anatomy and Histopathology, University of Siena, Italy.
FAU - Lazzi, Stefano
AU  - Lazzi S
FAU - Zazzi, Maurizio
AU  - Zazzi M
FAU - Lalinga, Anna Vittoria
AU  - Lalinga AV
FAU - Palummo, Nazareno
AU  - Palummo N
FAU - Galieni, Piero
AU  - Galieni P
FAU - Marafioti, Teresa
AU  - Marafioti T
FAU - Tonini, Tiziana
AU  - Tonini T
FAU - Cinti, Caterina
AU  - Cinti C
FAU - Leoncini, Lorenzo
AU  - Leoncini L
FAU - Pileri, Stefano A
AU  - Pileri SA
FAU - Tosi, Piero
AU  - Tosi P
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Receptors, Antigen, B-Cell)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/metabolism
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Gene Rearrangement, B-Lymphocyte, Heavy Chain/*genetics
MH  - Hodgkin Disease/*genetics/metabolism/pathology
MH  - Humans
MH  - Immunoglobulin Variable Region/*genetics/metabolism
MH  - Immunohistochemistry
MH  - Lymph Nodes/metabolism/pathology
MH  - Lymphoma, B-Cell/genetics/metabolism/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/genetics/metabolism/pathology
MH  - Lymphoma, Non-Hodgkin/*genetics/metabolism/pathology
MH  - Mutation
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, B-Cell/*genetics/metabolism
MH  - Reed-Sternberg Cells/*immunology/metabolism/pathology
MH  - Sequence Analysis, DNA
EDAT- 2002/02/21 10:00
MHDA- 2002/04/26 10:01
CRDT- 2002/02/21 10:00
PHST- 2002/02/21 10:00 [pubmed]
PHST- 2002/04/26 10:01 [medline]
PHST- 2002/02/21 10:00 [entrez]
AID - 10.1097/00019606-200203000-00002 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2002 Mar;11(1):2-8. doi: 10.1097/00019606-200203000-00002.