PMID- 11857507
OWN - NLM
STAT- MEDLINE
DCOM- 20020918
LR  - 20161124
IS  - 0040-3709 (Print)
IS  - 0040-3709 (Linking)
VI  - 65
IP  - 2
DP  - 2002 Feb
TI  - Effects of folate supplementation on the risk of spontaneous and induced neural 
      tube defects in Splotch mice.
PG  - 63-9
AB  - BACKGROUND: Neural tube defects (NTDs) are among the most common human congenital 
      malformations. Although clinical investigations have reported that 
      periconceptional folic acid supplementation can reduce the occurrence of these 
      defects, its mechanism remains unknown. Therefore, the murine mutant Splotch, 
      which has a high incidence of spontaneous NTDs, along with the inbred strains SWV 
      and LM/Bc, were used to investigate the relationship between folate and NTDs. 
      METHODS: To investigate whether folates could reduce spontaneous NTDs, 
      heterozygous Splotch dams (+/Sp) were treated with either folate or folinic acid 
      throughout neurulation, gestational day (GD) 6.5 to 10.5. On GD 18.5 the dams 
      were sacrificed and the fetuses examined for any neural tube defects. 
      Subsequently, Sp/+ dams were treated with arsenic while receiving either a folate 
      or folinic acid supplementation. Similar experiments were performed in the LM/Bc 
      and SWV strains. RESULTS: Neither folate nor folinic acid supplements reduced the 
      frequency of spontaneous NTDs in the embryos from Splotch heterozygote crosses. 
      Arsenic increased the frequency of NTDs and embryonic death in the Splotch, LM/Bc 
      and SWV litters and folinic acid failed to ameliorate the teratogenic effect of 
      this metal. A folate supplement given to arsenic-treated dams proved to be 
      maternally lethal in all three strains. CONCLUSIONS: Splotch embryos were not 
      protected from either spontaneous or arsenic-induced NTDs by folinic or folic 
      acid supplementation. Furthermore, folinic acid supplements did not reduce the 
      incidence of arsenic-induced NTDs in either the LM/Bc or SWV litters.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Gefrides, Lisa A
AU  - Gefrides LA
AD  - Department of Veterinary Anatomy and Public Heath, Texas A&M University, College 
      Station, Texas 77843-4458, USA.
FAU - Bennett, Gregory D
AU  - Bennett GD
FAU - Finnell, Richard H
AU  - Finnell RH
LA  - eng
GR  - DE11303/DE/NIDCR NIH HHS/United States
GR  - HD/ES35396/HD/NICHD NIH HHS/United States
GR  - P30 ES09106/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Teratology
JT  - Teratology
JID - 0153257
RN  - 0 (Arsenates)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (PAX3 Transcription Factor)
RN  - 0 (PAX3 protein, human)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (Teratogens)
RN  - 0 (Transcription Factors)
RN  - 138016-91-8 (Pax3 protein, mouse)
RN  - 935E97BOY8 (Folic Acid)
RN  - N7CIZ75ZPN (arsenic acid)
RN  - Q573I9DVLP (Leucovorin)
SB  - IM
MH  - Animals
MH  - Arsenates
MH  - DNA-Binding Proteins/physiology
MH  - Embryo Loss
MH  - Female
MH  - Fetal Resorption
MH  - Folic Acid/physiology/*therapeutic use
MH  - Genotype
MH  - Leucovorin/therapeutic use
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neural Tube Defects/genetics/*prevention & control
MH  - PAX3 Transcription Factor
MH  - Paired Box Transcription Factors
MH  - Teratogens
MH  - *Transcription Factors
EDAT- 2002/02/22 10:00
MHDA- 2002/09/19 10:01
CRDT- 2002/02/22 10:00
PHST- 2002/02/22 10:00 [pubmed]
PHST- 2002/09/19 10:01 [medline]
PHST- 2002/02/22 10:00 [entrez]
AID - 10.1002/tera.10019 [pii]
AID - 10.1002/tera.10019 [doi]
PST - ppublish
SO  - Teratology. 2002 Feb;65(2):63-9. doi: 10.1002/tera.10019.