PMID- 11858207 OWN - NLM STAT- MEDLINE DCOM- 20020816 LR - 20191105 IS - 0960-8524 (Print) IS - 0960-8524 (Linking) VI - 82 IP - 1 DP - 2002 Mar TI - Effect of pH on hydrogen production from glucose by a mixed culture. PG - 87-93 AB - The effect of pH on the conversion of glucose to hydrogen by a mixed culture of fermentative bacteria was evaluated. At 36 degrees C, six hours hydraulic retention, over 90% of glucose was degraded at pH ranging 4.0-7.0, producing biogas and an effluent comprising mostly fatty acids. At the optimal pH of 5.5, the biogas comprised 64 +/- 2% of hydrogen with a yield of 2.1 +/- 0.1 mol-H2/mol-glucose and a specific production rate of 4.6 +/- 0.4 l-H2/(g-VSS day). The effluent was composed of acetate (15.3-34.1%) and butyrate (31.2-45.6%), plus smaller quantities of other volatile fatty acids and alcohols. The diversity of microbial communities increased with pH, based on 16S rDNA analysis by denaturing gradient gel electrophoresis (DGGE). FAU - Fang, Herbert H P AU - Fang HH AD - Centre for Environmental Engineering Research, Department of Civil Engineering, University of Hong Kong. hrechef@hkucc.hku.hk FAU - Liu, Hong AU - Liu H LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 RN - 0 (Alcohols) RN - 0 (Fatty Acids, Volatile) RN - 0 (Sewage) RN - 7YNJ3PO35Z (Hydrogen) RN - IY9XDZ35W2 (Glucose) SB - IM MH - Alcohols/metabolism MH - Bacteria/metabolism MH - Biomass MH - Bioreactors/microbiology MH - Fatty Acids, Volatile/metabolism MH - Fermentation MH - Glucose/*metabolism MH - Hydrogen/*metabolism MH - Hydrogen-Ion Concentration MH - Sewage/*microbiology EDAT- 2002/02/23 10:00 MHDA- 2002/08/17 10:01 CRDT- 2002/02/23 10:00 PHST- 2002/02/23 10:00 [pubmed] PHST- 2002/08/17 10:01 [medline] PHST- 2002/02/23 10:00 [entrez] AID - S0960-8524(01)00110-9 [pii] AID - 10.1016/s0960-8524(01)00110-9 [doi] PST - ppublish SO - Bioresour Technol. 2002 Mar;82(1):87-93. doi: 10.1016/s0960-8524(01)00110-9.