PMID- 11858878
OWN - NLM
STAT- MEDLINE
DCOM- 20020927
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 20
IP  - 11-12
DP  - 2002 Feb 22
TI  - Absence of antigenic competition in Aotus monkeys immunized with Plasmodium 
      falciparum DNA vaccines delivered as a mixture.
PG  - 1675-80
AB  - Aotus lemurinus lemurinus monkeys were immunized four times with one of three DNA 
      plasmids expressing important Plasmodium falciparum blood stage vaccine candidate 
      proteins or with a mixture containing all three vaccines. The three vaccines 
      encoded sequences from apical merozoite antigen-1 (AMA-1), erythrocyte binding 
      protein-175 (EBA-175) and merozoite surface protein-1 (MSP-1). Antigen-specific 
      enzyme-linked immunosorbant assays (ELISAs) showed no significant differences in 
      antibody titer induced to the three antigens by a single vaccine compared with 
      the titer induced to that same antigen by the trivalent preparation. Results of 
      immunofluorescent antibody assays against erythrocytes infected with asexual 
      blood stage P. falciparum indicated that each of the three monovalent vaccines 
      induced significant antibody responses to whole parasites. The trivalent vaccine 
      mixture induced, after four immunizations, an antibody titer to whole parasites 
      that was 3--12-fold higher than those induced by any of the single vaccines. The 
      fourth immunization with the trivalent vaccine increased the mean antibody in 
      IFAT by more than five-fold.
FAU - Jones, Trevor R
AU  - Jones TR
AD  - Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver 
      Spring, MD 20910, USA. jonest@mmrc.navy.mil
FAU - Gramzinski, Robert A
AU  - Gramzinski RA
FAU - Aguiar, Joao C
AU  - Aguiar JC
FAU - Sim, B Kim Lee
AU  - Sim BK
FAU - Narum, David L
AU  - Narum DL
FAU - Fuhrmann, Steven R
AU  - Fuhrmann SR
FAU - Kumar, Sanjai
AU  - Kumar S
FAU - Obaldia, Nicanor
AU  - Obaldia N
FAU - Hoffman, Stephen L
AU  - Hoffman SL
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Carrier Proteins)
RN  - 0 (Malaria Vaccines)
RN  - 0 (Membrane Proteins)
RN  - 0 (Merozoite Surface Protein 1)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 0 (apical membrane antigen I, Plasmodium)
RN  - 0 (erythrocyte-binding antigen 175, Plasmodium)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/blood
MH  - Antigens, Protozoan/genetics
MH  - Aotus trivirgatus
MH  - Base Sequence
MH  - Carrier Proteins/genetics/immunology
MH  - Immunization
MH  - Immunization Schedule
MH  - Malaria Vaccines/*administration & dosage
MH  - Malaria, Falciparum/immunology/prevention & control
MH  - Membrane Proteins/genetics/immunology
MH  - Merozoite Surface Protein 1/genetics/immunology
MH  - Plasmids/genetics
MH  - Plasmodium falciparum/genetics/growth & development/*immunology
MH  - Protozoan Proteins/genetics/immunology
MH  - Vaccines, DNA/*administration & dosage
EDAT- 2002/02/23 10:00
MHDA- 2002/09/28 04:00
CRDT- 2002/02/23 10:00
PHST- 2002/02/23 10:00 [pubmed]
PHST- 2002/09/28 04:00 [medline]
PHST- 2002/02/23 10:00 [entrez]
AID - S0264410X01005138 [pii]
AID - 10.1016/s0264-410x(01)00513-8 [doi]
PST - ppublish
SO  - Vaccine. 2002 Feb 22;20(11-12):1675-80. doi: 10.1016/s0264-410x(01)00513-8.