PMID- 11859111
OWN - NLM
STAT- MEDLINE
DCOM- 20020315
LR  - 20201215
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 168
IP  - 5
DP  - 2002 Mar 1
TI  - Antigen-antibody immune complexes empower dendritic cells to efficiently prime 
      specific CD8+ CTL responses in vivo.
PG  - 2240-6
AB  - Dendritic cells (DCs) require a maturation signal to acquire efficient 
      CTL-priming capacity. In vitro FcgammaR-mediated internalization of Ag-Ab immune 
      complexes (ICs) can induce maturation of DCs. In this study, we show that 
      IC-induced DC maturation in vitro enables DCs to prime peptide-specific CD8+ CTLs 
      in vivo, independently of CD4+ Th cells. Importantly, OVA/anti-OVA IC-treated DCs 
      not only primed CD8+ CTLs to an exogenously loaded peptide nonrelated to OVA, but 
      also efficiently primed CTLs against the dominant CTL epitope derived from the 
      OVA Ag present in the ICs. Our studies show that ICs fulfill a dual role in 
      priming of CD8+ CTL responses to exogenous Ags: enhancement of Ag uptake by DCs 
      and activation of DCs, resulting in "license to kill." These findings indicate 
      that the presence of specific Abs can crucially affect the induction of cytotoxic 
      cellular responses.
FAU - Schuurhuis, Danita H
AU  - Schuurhuis DH
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Albinusdreef 2, 2300 RC Leiden, The Netherlands.
FAU - Ioan-Facsinay, Andreea
AU  - Ioan-Facsinay A
FAU - Nagelkerken, Bas
AU  - Nagelkerken B
FAU - van Schip, Jolien J
AU  - van Schip JJ
FAU - Sedlik, Christine
AU  - Sedlik C
FAU - Melief, Cornelis J M
AU  - Melief CJ
FAU - Verbeek, J Sjef
AU  - Verbeek JS
FAU - Ossendorp, Ferry
AU  - Ossendorp F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptides)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigen-Antibody Complex/*immunology
MH  - Cell Differentiation
MH  - Cell Line, Transformed
MH  - Cytotoxicity Tests, Immunologic
MH  - Dendritic Cells/*immunology
MH  - Endocytosis
MH  - Female
MH  - Histocompatibility Antigens Class I/physiology
MH  - Hybridomas
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Ovalbumin/immunology/metabolism
MH  - Peptides/immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2002/02/23 10:00
MHDA- 2002/03/16 10:01
CRDT- 2002/02/23 10:00
PHST- 2002/02/23 10:00 [pubmed]
PHST- 2002/03/16 10:01 [medline]
PHST- 2002/02/23 10:00 [entrez]
AID - 10.4049/jimmunol.168.5.2240 [doi]
PST - ppublish
SO  - J Immunol. 2002 Mar 1;168(5):2240-6. doi: 10.4049/jimmunol.168.5.2240.