PMID- 11859708
OWN - NLM
STAT- MEDLINE
DCOM- 20020510
LR  - 20061115
IS  - 0253-3766 (Print)
IS  - 0253-3766 (Linking)
VI  - 23
IP  - 6
DP  - 2001 Nov
TI  - [Localization and analysis of 1A6 gene by dual-color fluorescence in situ 
      hybridization on gastric carcinoma tissue and tumor cell lines].
PG  - 454-7
AB  - OBJECTIVE: To locate 1A6 gene on the chromosome and study its copy number by 
      dual-color fluorescence in situ hybridization (FISH) on three tumor cell lines 
      and 22 gastric tumors patients. METHODS: The single-color FISH of small size 
      biotin-labeled cDNA cloned in plasmid was used to locate the 1A6 gene on one of 
      the chromosomes in C group. Genomic DNA of 1A6 gene was screened by polymerase 
      chain reaction (PCR) in PAC library according to the sequence. The florenscence 
      of green and orange was incorporated into the target gene-1A6 cloned in PAC and 
      chromosome-12-specific alpha-satellite repeat DNA by nick translation, followed 
      by dual-color FISH. 1A6 gene was located in the metaphase chromosome of the 
      normal peripheral lymphocytes. 1A6 gene and chromosome 12 copy numbers were 
      analyzed on touch slide of gastric cancer tissue and cell lines. RESULTS: 1A6 
      gene was located in 12q23.2-23.3. The chromosome 12/1A6 signal ratio was 
      0.96-1.01 in breast, ovarian and gastric cancer cell lines. The ratio was 
      0.93-1.11 in gastric cancer tissue touch slides. The number of chromosome 12 is 
      disomic (87.7%), triploid (7.4%) in BMI cell line; multisomic(100%), including 
      pentasomic (67.6%) in SKOV3; the multisomic (83.1%), including trisomic (71%) in 
      SGC823. There are highly disomic rate in 86. 4% (19/22) of patients. CONCLUSION: 
      1A6 gene is in 12q23.2-23.3. Neither 1A6 amplification nor deletion in gastric 
      cancer tissue and three cell lines was found. Further study is needed for the 
      understanding of chromosome 12 copy number variation in different cancer cell 
      lines.
FAU - Zheng, S
AU  - Zheng S
AD  - Genetic Laboratory, Beijing Institute for Cancer Research, Beijing University 
      Institute of Oncology, Beijing 100034, China.
FAU - Ke, Y
AU  - Ke Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Zhong Liu Za Zhi
JT  - Zhonghua zhong liu za zhi [Chinese journal of oncology]
JID - 7910681
SB  - IM
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 12
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Stomach Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2002/02/28 10:00
MHDA- 2002/05/11 10:01
CRDT- 2002/02/28 10:00
PHST- 2002/02/28 10:00 [pubmed]
PHST- 2002/05/11 10:01 [medline]
PHST- 2002/02/28 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Zhong Liu Za Zhi. 2001 Nov;23(6):454-7.